Improvements in the Extractive and Carbohydrate Analysis of Sugarcane Bagasse
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ORIGINAL PAPER
Improvements in the Extractive and Carbohydrate Analysis of Sugarcane Bagasse Paula S. Barbosa1 · Marcio H. P. Barbosa2 · Bruno de F. H. de Faria3 · Reinaldo F. Teófilo1 Received: 19 May 2020 / Accepted: 30 September 2020 © Springer Nature B.V. 2020
Abstract Generic procedures for the determination of extractives and carbohydrates (glucose, xylose, galactose, arabinose, mannose) were improved specifically for sugarcane bagasse. The extraction time was optimized using a specific design of experiments. The optimal condition for sugarcane biomass with up to 20% extractives was 11.5 h of extraction in water, followed by 9 h in ethanol. For the carbohydrate analysis, a separation method using liquid chromatography was improved and optimized to reduce the retention time of furanic compounds. The reduction obtained was approximately 30%. The optimal condition was the isocratic elution of the mobile phase with an aqueous solution containing 15% acetonitrile. The percentages of glycans, xylans, and arabinans in the extractive-free biomass was 33%, 22%, and 6.5%, respectively. The galactans and mannans were not detected. Acetyl groups and uronic acids were not determined.
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s12649-020-01268-y) contains supplementary material, which is available to authorized users. * Reinaldo F. Teófilo [email protected] 1
Chemistry Department, Universidade Federal de Viçosa, Viçosa, MG 36570‑900, Brazil
2
Department of Plant Science, Universidade Federal de Viçosa, Viçosa, MG 36570‑900, Brazil
3
Department of Forestry Engineering, Universidade Federal de Viçosa, Viçosa, MG 36570‑900, Brazil
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Waste and Biomass Valorization
Graphic Abstract
Keywords Sugarcane · Holocellulose · Extractives · Lignocellulosic biomass · Carbohydrates Abbreviations GVL Gamma-valerolactone HMF 5-Hydroxymethylfurfural NREL National Renewable Energy Laboratory TAPPI Technical Association of the Pulp and Paper Industry ASTM American Society of Testing and Materials tw Extraction time in water te Extraction time in ethanol CCD Central composite design HPLC High-performance liquid chromatograph
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PDA Photodiode array ELSD Evaporative light scattering detector ACN Acetonitrile SRSs Sugar recovery standards BMT Biomass of the RB867515 variety, used as a test biomass for the development of the procedure BMT25 Biomass of the RB867515 variety, used as a test biomass for the development of the procedure, dried in open air
Waste and Biomass Valorization
BMT45 Biomass of the RB867515 variety, used as a test biomass for the development of the procedure, oven-dried at 45 °C for 24 h BMA32 Biomass of the RB127032 variety, used as a application biomass for the validation of the procedure BMA35 Biomass of the RB127035 variety, used as a application biomass for the validation of the procedure BMA39 Biomass of the RB127039 variety, used as a application biomass for the validation of the procedure BMA45 B
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