In focus in HCB
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EDITORIAL
In focus in HCB Douglas J. Taatjes1 · Jürgen Roth2 Published online: 16 September 2020 © Springer-Verlag GmbH Germany, part of Springer Nature 2020
Welcome to this month’s Editorial in which we highlight three original articles describing (1) the tissue localization of the molecular chaperone Mdg1/ERdj4 during chick embryonic development, (2) developmental patterns for ABC transporter protein expression in the brain of fetuses, neonates, and young children when compared to adults, and (3) the role of the cyclic AMP response element-binding protein (CREB) in mouse primordial follicle activation. In addition, we highlight one short communication presenting a new immunohistochemical protocol to identify skeletal muscle fiber subtypes, the basal lamina, and capillaries in a single tissue section. As always, we hope you enjoy perusing all of the articles in this issue as the summer season winds to an end.
(2001) have reported that this cochaperone is transiently upregulated in differentiating microvascular endothelial cells. In their present investigation (Daverkausen‑Fischer et al. 2020), they have analyzed Mdg1/ERdj4 during chick embryonic development by immunohistochemistry. In the paraxial and lateral plate mesoderm of chick embryos at day 2 (HH stage 12 and 13), immunoreactivity for Mdg1/ERdj4 was evenly distributed in all mesenchymal cells (Fig. 1).
Molecular chaperone Mdg1/ERdj4 expression during chick development Molecular chaperones, also called helper proteins, play a fundamental role in maintaining a functional proteome by assisting proteins to fold properly and thereby to prevent protein aggregation (Kim et al. 2013). One group of molecular chaperones is the Hsp70 system and its cycle is regulated by Hsp40 cochaperones. Besides their role in protein folding, molecular chaperones and cochaperones may have other functions as indicated by their presence in specific tissues and their regulated expression during development (Angelier et al. 1996; Vega-Nunez et al. 1999; Vos et al. 2008). Mdg1/ ERdj4 is a vertebrate-specific cochaperone located in different cellular organelles (Berger et al. 2003; Dong et al. 2008; Pröls et al. 2001; Shen et al. 2002). Previously, Pröls et al. * Douglas J. Taatjes [email protected] 1
Department of Pathology and Laboratory Medicine, Larner College of Medicine, University of Vermont, Burlington, VT 05405, USA
University of Zurich, CH‑8091 Zurich, Switzerland
2
Fig. 1 Immunohistochemical Mdg1/ERdj4 protein pattern in the paraxial mesoderm. From Daverkausen-Fischer et al. (2020)
During mesenchymal-epithelial transition, immunostaining for Mdg1/ERdj4 became localized and concentrated in the apical and basal zones of the epithelial linings. In sclerotomal cells, after epithelial-mesenchymal transition, the apical-basal pattern of Mdg1/ERdj4 changed to the diffuse mesenchymal pattern. The detailed analysis of the developing central nervous system and the digestive tract revealed the epithelia-specific apical-basal and mesenchyme-specific diffuse Mdg1/ERd
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