In Silico Engineering of Disulphide Bonds to Produce Stable Cellulase
This Brief highlights different approaches used to create stable cellulase and its use in different fields. Cellulase is an industrial enzyme with a broad range of significant applications in biofuel production and cellulosic waste management. Cellulase 7
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Bahram Barati Iraj Sadegh Amiri
In Silico Engineering of Disulphide Bonds to Produce Stable Cellulase 123
SpringerBriefs in Applied Sciences and Technology
More information about this series at http://www.springer.com/series/8884
Bahram Barati Iraj Sadegh Amiri •
In Silico Engineering of Disulphide Bonds to Produce Stable Cellulase
123
Bahram Barati University Technology Malaysia Skudai Malaysia
Iraj Sadegh Amiri University of Malaya Kuala Lumpur Malaysia and Laser Center, Ibnu Sina ISIR Universiti Teknologi Malaysia (UTM) Skudai, Johor Bahru Malaysia
ISSN 2191-530X ISSN 2191-5318 (electronic) SpringerBriefs in Applied Sciences and Technology ISBN 978-981-287-431-3 ISBN 978-981-287-432-0 (eBook) DOI 10.1007/978-981-287-432-0 Library of Congress Control Number: 2015931419 Springer Singapore Heidelberg New York Dordrecht London © The Author(s) 2015 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. Printed on acid-free paper Springer Science+Business Media Singapore Pte Ltd. is part of Springer Science+Business Media (www.springer.com)
Preface
Cellulase is an industrial enzyme which has significant applications in biofuel production and cellulosic waste management. Cellulase 7a from Trichoderma reesei is the most efficient enzyme in biohydrolysis of cellulose. In order to improve its thermostability it can be engineered by many approaches, such as hydrophobic interactions, aromatic interactions, hydrogen bonds, ion pairs, and disulfide bridge creation. In this study, introduction of disulfide bonds into the enzyme was chosen as an approach to achieve this aim. According to disulfide by design software, potential residues for creating disulfide bonds were identified. Accordingly, nine residues are mutated to cysteine, and as a result, five disulfide bridges were created. In order to study conformational stability of all the mutated proteins, molecular dynamic simulation was run for 20,000 ps by using GROMACS software. Rootmean-square deviation (RMSD), Root-mean-square fluct
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