In vitro cultivation of tansy ( Tanacetum vulgare L.): a tool for the production of potent pharmaceutical agents
- PDF / 3,203,037 Bytes
- 13 Pages / 595.276 x 790.866 pts Page_size
- 94 Downloads / 169 Views
ORIGINAL ARTICLE
In vitro cultivation of tansy (Tanacetum vulgare L.): a tool for the production of potent pharmaceutical agents Nina Devrnja 1 Dušica Ćalić 1
&
Dijana Krstić-Milošević 1 & Dušica Janošević 2 & Vele Tešević 3 & Branka Vinterhalter 1 & Jelena Savić 1 &
Received: 11 September 2020 / Accepted: 18 November 2020 # Springer-Verlag GmbH Austria, part of Springer Nature 2020
Abstract In this study, tansy (Tanacetum vulgare L.) in vitro culture was established from seeds collected from natural populations. The multiplication of plantlets was conducted through shoot tips that exhibited potent apical growth and regeneration capacities on basal medium (BM), without the addition of any plant growth regulators (PGRs). PGRs were also omitted for the establishment and cultivation of tansy root cultures. Both abaxial and adaxial leaf surfaces of in vitro micropropagated plantlets were covered with glandular biseriate trichomes. Histochemical staining showed that glandular secretions were rich in lipid and terpene compounds, confirmed by GC-MS analysis of essential oil (EO). In the total EO, similar portions of oxygenated monoterpenes (38.5% m/m) and oxygenated sesquiterpenes (22.6% m/m) were detected. Chemical profiles of methanol extracts of in vitro cultured tansy shoots and roots varied in quantity and quality from those obtained from wild-growingtansy. HPLC analysis indicated that the methanol extracts of in vitro cultured roots were the richest in 3,5-O-dicaffeoylquinic acid (3,5-O-DCQA), in which the concentration was 6 times higher (10.220 mg/g DW) than that in the extract obtained from roots of wild-growing tansy (1.684 mg/g DW). This result is noticeable in the manner of industrial production of biologically active 3,5-O-DCQA that has been shown to have antioxidant, hepatoprotective, antiviral, antimutagenic, and immunomodulatory activity. Biotechnological interventions on secondary metabolite production taking place in trichomes could further enhance the production of some important tansy metabolites and further investigation will be directed toward the elucidation of the pharmaceutical potential of tansy in vitro obtained metabolites, as mixtures or single moieties. Keywords Tansy . In vitro cultivation . Essential oil . Methanol extracts . Histochemical analysis . Phytochemical analysis
Introduction The current pharmacological industry mostly relies on synthetic drugs, due to the intensive development of a computational approach, combinatorial chemistry techniques, and Handling Editor: Handling Editor: Peter Nick * Nina Devrnja [email protected] 1
Institute for Biological Research “Siniša Stanković”-National Institute of Republic of Serbia, University of Belgrade, Bulevar despota Stefana 142, Belgrade 11060, Serbia
2
Faculty of Biology, University of Belgrade, Studentski trg 16, Belgrade, Serbia
3
Faculty of Chemistry, University of Belgrade, Studentski trg 16, Belgrade, Serbia
instruments during the second half of the twentieth century (Liu et al. 2017). Despite what was expected,
Data Loading...
