In vitro culture of Keratinocytes from human umbilical cord blood mesenchymal stem cells: the Saigonese culture
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In vitro culture of Keratinocytes from human umbilical cord blood mesenchymal stem cells: the Saigonese culture Tran Cong Toai • Huynh Duy Thao • Ciro Gargiulo Nguyen Phuong Thao • Tran Thi Thanh Thuy • Huynh Minh Tuan • Nguyen Thanh Tung • Luis Filgueira • D. Micheal Strong
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Received: 6 January 2010 / Accepted: 3 March 2010 / Published online: 27 March 2010 Ó Springer Science+Business Media B.V. 2010
Abstract There have been many attempts to acquire and culture human keratinocytes for clinical purposes including from keratotome slices in media with fetal calf serum (FCS) or pituitary extract (PE), from skin specimens in media with feeder layers, from suction blister epidermal roofs’ in serum-free culture and from human umbilical cord blood (hUCB) mesenchymal stem cells (MSCs) in media with skin feeder layers. Conversely this study was designed to investigate whether keratinocytes could be obtained directly from hUCB MSCs in vitro. It is widely established that mesenchymal stem cells from human umbilical cord blood have multipotent capacity and the ability to differentiate into disparate cell lineages hUCB MSCs T. C. Toai (&) H. D. Thao N. P. Thao T. T. T. Thuy H. M. Tuan Department of Histo-pathology, Embryology, Genetics and Biotechnology for Tissue Transplants, Pham Ngoc Thach Medical University, Ho Chi Minh City, Vietnam e-mail: [email protected] C. Gargiulo L. Filgueira University of Western Australia School of Anatomy and Human Biology, Crawley, WA, Australia D. M. Strong Department of Orthopaedics and Sport Medicine, University of Washington School of Medicine, Seattle, WA, USA N. T. Tung Department of Pathology, Children No 1 Hospital in HCMC, Ho Chi Minh City, Vietnam
were directly induced to differentiate into keratinocytes by using a specific medium composed of primary culture medium (PCM) and serum free medium (SFM) in a ratio 1:9 for a period of 7 days and tested by immunostain p63 and K1-K10. Cells thus cultured were positive in both tests, confirming the possibility to directly obtain keratinocytes from MSCs hUCB in vitro. Keywords Mesenchymal stem cell UCB Keratinocyte culture Cell culture
Introduction MSCs from human-UCB Human UCB is a significant source of hematopoietic stem cells and has been considered as a valid alternative for hematopoietic stem cell transplantation (Toai et al. 2009; Lee et al. 2004; Park et al. 2006; Van de Ven et al. 2007; Maurice et al. 2007; Musina et al. 2007; Sasaki et al. 2008). MSCs from hUCB have been used in a wide range of diseases such as liver disorders, myocardial infarction, central nervous system condition or in degenerative pathologies such as diabetes, Crohn’s disease, osteogenesis imperfect (OI), rheumatoid arthritis (RA) and osteoarthritis (OA) (Toai et al. 2009; Lee et al. 2004;
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Riordan et al. 2007; Kogler and Wernet 2006; Kim et al. 2004; Reddi 2007; Koblas et al. 2005; De Bari and Dell’Accio 2007; Tuan and Chen 2006; Waese and Kandel 2007; Park et al. 2006). The most valuable potential of MSCs is their ability to switch i
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