Human umbilical cord-derived MSC culture: the replacement of animal sera with human cord blood plasma
- PDF / 494,976 Bytes
- 7 Pages / 595.276 x 790.866 pts Page_size
- 25 Downloads / 218 Views
man umbilical cord-derived MSC culture: the replacement of animal sera with human cord blood plasma Yan Ding & Hua Yang & Jing Bo Feng & Ying Qiu & Dong Sheng Li & Yi Zeng
Received: 3 April 2013 / Accepted: 7 July 2013 / Published online: 17 September 2013 / Editor: T. Okamoto # The Society for In Vitro Biology 2013
Abstract Human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) hold great potential for their therapeutic use in various clinical diseases. Many publications have reported on human blood-derived alternatives to animal serum for culturing mesenchymal stem cells, such as human serum, allogenic umbilical cord blood serum, and human platelet derivatives. However, it is not clear whether human umbilical cord blood plasma (UCBP), as the surplusage of umbilical cord blood mesenchymal stem cell extraction, could be used. In this study, in order to make the best of umbilical cord blood, the human UCBP was dialyzed to replace fetal bovine serum (FBS) in the culture medium. hUC-MSCs were cultured in the new medium. Cell growth rate, specific biomarkers, and differentiation properties were detected to characterize the cell proliferation and MSC-specific properties. The hUC-MSCs cultured in such derived medium were verified with proliferation rate, cluster differentiation markers, cell cycle, as well as differentiation capabilities. Such dialyzed human UCBP is fully comparable with, if not superior to, FBS in deriving and culturing hUC-MSCs. Keywords Fetal bovine serum . Mesenchymal stem cell . Umbilical cord blood . Growth factor Y. Ding : Y. Zeng (*) College of Life Science and Bioengineering, Beijing University of Technology, Beijing 100124, China e-mail: [email protected] Y. Ding : J. B. Feng : D. S. Li (*) Hubei Key Laboratory of Embryonic Stem Cell Research, Taihe Hospital, Hubei University of Medicine, Shiyan, Hubei 442000, China e-mail: [email protected] H. Yang : Y. Qiu Reproductive Medical Center of Nanning Second People’s Hospital, Third Affiliated Hospital of Guangxi Medical University, Nanning 530031, China
Introduction Human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) are a group of adult stem cells that originated from Wharton's jelly of the umbilical cord. They are multipotent and have the ability of self-renewal. hUCMSCs possess great plasticity and are able to differentiate into a variety of cell types, including bone, cartilage, adipose tissue, and tendon (Wu et al. 2007; Karahuseyinoglu et al. 2008; Pereira et al. 2008). Under appropriate conditions, they can even transdifferentiate into other germ layer cells, such as skeletal cells, myocardial cells, hepatocytes, and neural cells (Grove et al. 2004). Their therapeutic benefits have been demonstrated in various neuro-dysfunction animal models, such as ischemic stroke, Parkinsonism, and spinal cord injury (Ding et al. 2007; Yang et al. 2008). Conventional media used for culturing hUC-MSCs are typically supplemented with xenogenic supplements such as fetal bovine serum (FBS). However, xenogeneic proteins and the pres
Data Loading...
