In vitro evaluation of potential prebiotic effects of a freeze-dried juice from Pilosocereus gounellei (A. Weber ex K. S
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ORIGINAL ARTICLE
In vitro evaluation of potential prebiotic effects of a freeze‑dried juice from Pilosocereus gounellei (A. Weber ex K. Schum. Bly. Ex Rowl) cladodes, an unconventional edible plant from Caatinga biome Thais Santana Ribeiro1 · Karoliny Brito Sampaio2 · Francisca Nayara Dantas Duarte Menezes2 · Paloma Oliveira Antonino de Assis1 · Marcos dos Santos Lima3 · Maria Elieidy Gomes de Oliveira1 · Evandro Leite de Souza2,4 · Rita de Cássia Ramos do Egypto Queiroga1 Received: 13 June 2020 / Accepted: 15 September 2020 / Published online: 24 September 2020 © King Abdulaziz City for Science and Technology 2020
Abstract This study evaluated in vitro the potential prebiotic effects of a freeze-dried juice extracted from cladodes of Pilosocereus gounellei (A. Weber ex K. Schum.) Bly. Ex Rowl, an unconventional edible plant from Brazilian Caatinga biome and popularly known as xique-xique. Prebiotic effects of freeze-dried xique-xique cladode juice (XCJ, 20 g/L) were evaluated by measurements of prebiotic activity scores and stimulatory effects on growth and metabolic activities of probiotic Lactobacillus acidophilus LA-05, L. casei L-26 and L. paracasei L-10, which are beneficial species found as part of human gut microbiota. XCJ showed positive prebiotic activity scores on all examined probiotics, indicating a selective stimulatory effect on these microorganisms in detriment to enteric pathogens. Examined probiotics had high viable counts (> 8 log CFU/mL) after 48 h of cultivation in media with XCJ (20 g/L), representing an increase of > 2 log CFU/mL when compared to viable counts found on time zero. Cultivation of probiotics in media with XCJ resulted in decreased pH during the 48 h-incubation. Contents of fructose and glucose decreased in media with XCJ inoculated with L. acidophilus LA-05, L. casei L-26 or L. paracasei L-10 during the 48 h-cultivation, in parallel with an increase in contents of acetic and lactic acids. Measured effects of XCJ on probiotics were overall similar to those exerted by fructoligosaccharides (20 g/L), a proven prebiotic ingredient. These results showed that XCJ could exert selective stimulatory effects on different Lactobacillus species, which are indicative of potential prebiotic properties. Keywords Cactaceae · Prebiotic · Probiotic · Metabolic activity · Valorization
Introduction
* Evandro Leite de Souza [email protected] 1
Laboratory of Bromatology, Department of Nutrition, Health Sciences Center, Federal University of Paraíba, João Pessoa, Paraíba, Brazil
2
Laboratory of Food Microbiology, Department of Nutrition, Health Sciences Center, Federal University of Paraíba, João Pessoa, Paraíba, Brazil
3
Department of Food Technology, Federal Institute of Sertão de Pernambuco, Petrolina, Petrolina, Brazil
4
Laboratório de Microbiologia de Alimentos, Departamento de Nutrição, Centro de Ciências da Saúde, Universidade Federal da Paraíba, Campus I, Cidade Universitária, João Pessoa, Paraíba 58051‑900, Brazil
Interest in rational explotaiton of plant bio
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