Evaluation of Probiotic Properties and Prebiotic Utilization Potential of Weissella paramesenteroides Isolated From Frui
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Evaluation of Probiotic Properties and Prebiotic Utilization Potential of Weissella paramesenteroides Isolated From Fruits Kinjal Pabari 1,2 & Sheetal Pithva 3 & Charmy Kothari 1 & Ravi Kiran Purama 4 & Kanthi Kiran Kondepudi 5 & Bharatkumar Rajiv Manuel Vyas 2 & Ramesh Kothari 2 & Padma Ambalam 1
# Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract Weissella paramesenteroides has gained a considerable attention as bacteriocin and exopolysaccharide producers. However, potential of W. paramesenteroides to utilize different prebiotics is unexplored area of research. Fruits being vectors of various probiotics, five W. paramesenteroides strains, namely, FX1, FX2, FX5, FX9, and FX12, were isolated from different fruits. They were screened and selected based on their ability to survive at pH 2.5 and in 1.0% sodium taurocholate, high cell surface hydrophobicity, mucin adhesion, bile-induced biofilm formation, antimicrobial activity (AMA) against selected enteropathogens, and prebiotic utilization ability, implicating the functional properties of these strains. In vitro safety evaluation showed that strains were susceptible to antibiotics except vancomycin and did not harbor any virulent traits such as biogenic amine production, hemolysis, and DNase production. Based on their functionality, two strains FX5 and FX9 were selected for prebiotic utilization studies by thin layer chromatography (TLC) and short-chain fatty acids (SCFAs) production by high performance liquid chromatography. TLC profile evinced the ability of these two strains to utilize low molecular weight galactooligosaccharides (GOS) and fructooligosaccharides (FOS), as only the upper low molecular weight fractions were disappeared from cell-free-supernatants (CFS). Enhanced β-galactosidase activity correlated with galactose accumulation in residual CFS of GOS displayed GOS utilization ability. Both the strains exhibited AMA against E. coli and Staph. aureus and high SCFAs production in the presence of prebiotic, suggesting their synbiotic potential. Thus, W. paramesenteroides strains FX5 and FX9 exhibit potential probiotic properties with prebiotic utilization and can be taken forward to evaluate synergistic synbiotic potential in detail. Keywords Weissella paramesenteroides . Probiotics . Prebiotics . Antimicrobial activity . Short-chain fatty acids
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s12602-019-09630-w) contains supplementary material, which is available to authorized users. * Ramesh Kothari [email protected] * Padma Ambalam [email protected] 1
Department of Biotechnology, Christ College, Vidya Niketan, Saurashtra University, PO, Rajkot, Gujarat 360005, India
2
UGC-CAS Department of Biosciences, Saurashtra University, Rajkot, Gujarat 360005, India
3
Government Science College, Sector 15, Gandhinagar, India
4
National Institute of Plant Genome Research, Aruna Asaf Marg, Po Box No. 10531, New Delhi, India
5
National Agri-Food Biotechnology Institute, S.A.S
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