In vitro tissue culture protocol of ancient einkorn ( Triticum monococcum ssp. monococum ) wheat via indirect shoot rege
- PDF / 4,005,610 Bytes
- 9 Pages / 595.276 x 790.866 pts Page_size
- 78 Downloads / 218 Views
PLANT TISSUE CULTURE
In vitro tissue culture protocol of ancient einkorn (Triticum monococcum ssp. monococum) wheat via indirect shoot regeneration Mehmet Örgeç 1 & Sandeep Kumar Verma 2 & Günce Şahin 1 & Nusret Zencirci 1 & Ekrem Gürel 1 Received: 17 August 2020 / Accepted: 3 September 2020 / Editor: Tom Clemente # The Society for In Vitro Biology 2020
Abstract Einkorn indirect shoot regeneration was obtained from leaf, coleoptile, and root explants cultured on full, ½, and ¼ strength Murashige and Skoog (MS) medium containing 0.5 to 10 mg L−1 2,4-dichlorophenoxyacetic acid (2,4-D). Calluses derived from coleoptile and root explants were cultured on full-strength MS medium supplemented with 0.5 to 5 mg L−1 thidiazuron (TDZ) for 6 wk to induce plant regeneration. Only callus cultures derived from coleoptile explants regenerated shoots. For root induction, 1.0 to 10 mg L−1 indole-3-acetic acid (IAA) was used for 4 wk. The highest rate of callus formation (93%) was from root explants cultured on full-strength MS medium with 4 mg L−1 2,4-D. Coleoptile explants had the highest callus formation (100%) after culture on full-strength MS medium with 3 to 6 mg L−1 of 2,4-D. The highest indirect shoot regeneration (7.0 ± 1.1 shoots produced per callus with a 67% shoot formation frequency) was from calluses derived from coleoptile explant cultured on full MS medium supplemented with 5 mg L−1 of TDZ. Of the different IAA concentrations investigated for rooting, the greatest number of roots per explant (7.7 ± 0.088 roots produced per regenerated shoot with a 100% root formation frequency) was observed on MS medium supplemented with 6 mg L−1 IAA. This indirect shoot regeneration protocol for coleoptile-derived einkorn wheat callus will be useful for future wheat genetic and improvement studies. Keywords Einkorn wheat . Callus . Regeneration . Coleoptile . Explant
Introduction One of the main crops grown worldwide for consumption and production is wheat (Triticum aestivum L.). It is a member of Poaceae family, and it is an important nutrient source which supplies energy as food/feed for human and animals. This is why it continues to be a strategic and indispensable food source for people throughout history. The human population is estimated to rise around 9.15 billion in 2050 (Alexandratos and Bruinsma 2012). Feeding a growing population will be one of the biggest problems facing humankind in the future. In addition to this, developing
* Nusret Zencirci [email protected] 1
Department of Biology, Faculty of Science and Art, Bolu Abant İzzet Baysal University, Gölköy, 14200 Bolu, Turkey
2
Institute of Biological Science, SAGE University, Kailod Kartal, Rau Bypass Road, Indore (M.P.), India
plants capable of adequate yields when grown under various stress conditions (cold, drought, salt, diseases, and micromacro elements toxicity) is, unfortunately, a serious challenge today. To solve these problems, new approaches should be developed to improve the quality and quantity of crop yields grown under a variety of environmental
Data Loading...
