Efficient plant regeneration from in vitro leaves and petioles via shoot organogenesis in Sapium sebiferum Roxb.
- PDF / 1,993,047 Bytes
- 14 Pages / 595.276 x 790.866 pts Page_size
- 65 Downloads / 193 Views
ORIGINAL ARTICLE
Efficient plant regeneration from in vitro leaves and petioles via shoot organogenesis in Sapium sebiferum Roxb. Jinyan Hou1 · Pengfei Su2 · Dacheng Wang2 · Xue Chen1 · Weiwei Zhao1 · Lifang Wu1,2,3 Received: 19 January 2020 / Accepted: 8 May 2020 © Springer Nature B.V. 2020
Abstract Sapium sebiferum Roxb. is a widespread and economically important multipurpose tree due to its high value in ornamental, and biodiesel production as well as medicine. A highly efficient in vitro plant regeneration system through direct shoot organogenesis was established for the first time from leaves and petioles of S. sebiferum. The results showed that plant growth regulators (PGRs), mechanical damage, explant orientation, explant source, and developmental stage had a strong influence on the in vitro morphogenesis of S. sebiferum. For shoot organogenesis from leaves, the highest adventitious shoot induction rate (96.67%) with 25.67 shoots per explant was obtained when mechanically damaged leaves (the first three leaf explants at the top, leaf #1–3) were cultured with the abaxial surface placed down on Murashige and Skoog (MS) medium containing 0.5 mg L −1 thidiazuron (TDZ). For in vitro morphogenesis of petioles, the combination of 1-naphthylacetic acid (NAA) and 6-benzylainopurine (6-BA) played a key role in cell fate determination. All of the in vitro petioles produced adventitious shoots on MS medium containing 1.0 mg L −1 6-BA and 0.1 mg L −1 NAA, while they produced green calli on −1 −1 medium fortified with 0.5 mg L 6-BA and 1.0 mg L NAA. The shoots were subcultured in medium fortified with 0.5 mg −1 −1 L 6-BA and 0.1 mg L NAA for multiplication and elongation. The elongated shoots successfully rooted on half-strength MS (1/2 MS) medium fortified with 0.5 mg L −1 indole-butyric acid (IBA) and 0.25 mg L −1 indole-3-acetic acid (IAA), and the regenerated plantlets successfully acclimatized with a survival rate of 92.56% in the greenhouse. The genetic fidelity of in vitro regenerated plants was evaluated using inter simple sequence repeat molecular markers. The in vitro regenerated plants were found to be the true to their mother plant. This study will be beneficial for the large-scale propagation as well as the genetic improvement of S. sebiferum. Keywords Shoot organogenesis · Sapium sebiferum · Leaf · Petiole · Developmental stage · Genetic stability Abbreviations 6-BA 6-Benzylainopurine 1/2 MS Half-strength Murashige and Skoog medium IAA Indole-3-acetic acid IBA Indole-butyric acid Communicated by M. Paula Watt. * Lifang Wu [email protected] 1
Key Laboratory of High Magnetic Field and Ion Beam Physical Biology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei 230031, Anhui, People’s Republic of China
2
School of Life Science, Anhui University, Hefei 230039, Anhui, People’s Republic of China
3
Taihe Experimental Station, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Taihe 236626, Anhui, People’s Republic of China
ISSR Inter-simple seq
Data Loading...
