In vivo preclinical PET/CT imaging of carbon-11-labeled aminoglycerol probe for the diagnosis of liver fibrosis
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ORIGINAL ARTICLE
In vivo preclinical PET/CT imaging of carbon‑11‑labeled aminoglycerol probe for the diagnosis of liver fibrosis Xi Chen1 · Xin Zhang1 · Ming Du1 · Chengyan Dong2 · Li Cao1 · Rucheng Wei1 · Changping Liu1 · Wei Zhai1 · Bo Wang1 · Jun Xin1 Received: 10 March 2019 / Accepted: 30 July 2019 © The Japanese Society of Nuclear Medicine 2019
Abstract Objective As an important membrane protein, aquaglyceroporin involves liver glycerol metabolism, which can be used to stage liver fibrosis. In this study, we synthesized a novel molecular probe carbon-11-labeled AR ([11C]AR) with aminoglycerol (AR), and evaluated its preclinical performance for liver fibrosis diagnosis by positron emission tomography/computed tomography (PET/CT) imaging in vivo. Methods We developed a fully automatic synthesis procedure for the preparation of [ 11C]AR by radiolabeling glycerol analogue precursor AR with carbon-11. The liver uptake kinetics of [ 11C]AR was investigated using a rat model by the PET/CT scanner. The dynamic PET/CT scans were performed between the control group (n = 5) and experimental group (n = 25), which was divided into three subgroups (S1, S2 + S3, S4) based on the stages of liver fibrosis. The regions of interest (ROIs) of 20 pixels were drawn in the liver area on the reconstructed images. One-way analysis of variance and independent sample t test were used to analyze the statistical difference of the maximum standardized uptake value (SUVmax) among the groups at series of scanning time points (20 s, 60 s, 90 s, 150 s, 5 min, 10 min, 20 min and 25 min). Results The fully automatic synthesis of [11C]AR was successfully achieved with high synthesis efficiency (above 50%). The uptake of [11C]AR in progressive liver fibrosis tissues was significantly lower than that in healthy livers at all the imaging time points (P 70%.
Statistical analysis The results were expressed as mean ± standard deviation. Independent sample t test was used to compare SUVmax of different groups at series of scanning time points, between control and experimental groups with different stages of liver fibrosis, respectively. P
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