Incorporation of Hydroxyapatite/Doxorubicin into the Chitosan/Polyvinyl Alcohol/Polyurethane Nanofibers for Controlled R
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ISSN 1229-9197 (print version) ISSN 1875-0052 (electronic version)
Incorporation of Hydroxyapatite/Doxorubicin into the Chitosan/Polyvinyl Alcohol/Polyurethane Nanofibers for Controlled Release of Doxurubicin and Its Anticancer Property Sayyed Sadroddin Qavamnia1*, Leila Roshanfekr Rad2, and Mohammad Irani3* 1
Textile Engineering Department, Birjand Branch, Islamic Azad University, Birjand 9717711111, Iran Department of Chemistry, Guilan Science and Research Branch, Islamic Azad University, Guilan, Iran 3 Faculty of Pharmacy, Alborz University of Medical Sciences, Karaj, Iran (Received July 26, 2019; Revised November 26, 2019; Accepted December 6, 2019)
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Abstract: In the present study, the doxorubicin-hydroxyapatite (DOX-HAp) has been loaded into the chitosan/PVA/PU single layer and core-shell nanofibers. The potential of synthesized nanofibers was evaluated for controlled release of DOX and bone cancer treatment in vitro. The synthesized DOX-HAp nanohybrid was characterized using XRD, SEM, and UV-Vis analysis. The morphology of synthesized nanofibers was examined by SEM and TEM analysis. For single layer nanofibers, some DOX-HAp nanohybrids were observed on the nanofibers surface. The fiber diameter was increased by increasing shell flow rate and no DOX-HAp nanohybrids were detected on the core-shell fibers surface. The DOX encapsulation efficiency of single layer and core-shell layer fibers was higher than 90 %. The initial burst release from single layer fiber at initial hours and the continuous release of DOX was observed from single layer nanofibers during 7 and 10 days under acidic and physiological pH. The sustained release of DOX was obtained within 10 and 14 days, 15 and 18 days, 21 and 25 days from core-shell fibers with flow rates of 0.3, 0.5 and 0.8 ml/h under acidic and physiological pH. The non-Fickian diffusion and Fickian diffusion were achieved from single layer and core-shell nanofibers. The cell attachment and cell death results indicated the high potential of DOX loaded-core-shell fibers for bone cancer treatment. Keywords: Hydroxyapatite, Chitosan, Core-shell nanofibers, Doxurubicin, Bone cancer
drugs [22]. However, the electrospinning of chitosan due to instability of jet solution is difficult. Different synthetic polymers such as polyvinyl alcohol (PVA), polyethylene oxide (PEO), polyurethane (PU), etc. were blended with chitosan solution to facilitate its electrospinning for preparation of uniform fibers [23]. Various bioactive materials such as bioactive glasses [2427], hydroxyapatite (HAp) [28], scaffolds [29], etc. have been developed for bone tissue regeneration. HAp (Ca10 (PO4)6 (OH)2) nanoparticles as a mineral biocompatible and osteoconductive matrix have been synthesized from various methods such as sol-gel, precipitation, hydrothermal etc. [28] and used for bone regeneration and delivery systems of genes, proteins, and drugs for various cancer treatment such as bone cancer [30,31], breast cancer [32,33] etc. Furthermore, the controlled release of drugs for cancer treatment cou
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