Increase in number of circulating disseminated epithelial cells after surgery for non-small cell lung cancer monitored b
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Increase in number of circulating disseminated epithelial cells after surgery for non-small cell lung cancer monitored by MAINTRAC® is a predictor for relapse: A preliminary report Axel Rolle1, Rainer Günzel1, Ulrich Pachmann2, Babette Willen2, Klaus Höffken3 and Katharina Pachmann*2,3 Address: 1Fachkrankenhaus Coswig/Dresden, der Friedrich-Schiller-Universität Jena, Germany, 2TZB Transfusionsmedizinisches Zentrum Bayreuth, der Friedrich-Schiller-Universität Jena, Germany and 3Klinik für Innere Medizin II der Friedrich-Schiller-Universität Jena, Germany Email: Axel Rolle - [email protected]; Rainer Günzel - [email protected]; Ulrich Pachmann - [email protected]; Babette Willen - [email protected]; Klaus Höffken - [email protected]; Katharina Pachmann* - [email protected] * Corresponding author
Published: 31 March 2005 World Journal of Surgical Oncology 2005, 3:18
doi:10.1186/1477-7819-3-18
Received: 29 November 2004 Accepted: 31 March 2005
This article is available from: http://www.wjso.com/content/3/1/18 © 2005 Rolle et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract Background: Lung cancer still remains one of the most commonly occurring solid tumors and even in stage Ia, surgery fails in 30% of patients who develop distant metastases. It is hypothesized that these must have developed from occult circulating tumor cells present at the time of surgery, or before. The aim of the present study was to detect such cells in the peripheral blood and to monitor these cells following surgery. Methods: 30 patients treated for lung cancer with surgery were monitored for circulating epithelial cells (CEC) by taking peripheral blood samples before, 2 weeks and 5 months after surgery and/or radiotherapy (RT) chemotherapy (CT) or combined RT/CT using magnetic bead enrichment and laser scanning cytometry (MAINTRAC®) for quantification of these cells. Results: In 86% of the patients CEC were detected before surgery and in 100% at 2 weeks and 5 months after surgery. In the control group, which consisted of 100 normal donors without cancer, 97 % were negative for CEC. A significantly higher number of CEC was found preoperatively in patients with squamous cell carcinoma than in those with adenocarcinoma. In correlation to the extent of parenchymal manipulation 2 weeks after surgery, an increase in numbers of CEC was observed with limited resections (18/21) whereas pneumonectomy led to a decrease (5/8) of CEC, 2 weeks after surgery. The third analysis done 5 months after surgery identified 3 groups of patients. In the group of 5 patients who received neoor adjuvant chemo/radiotherapy there was evidence that monitoring of CEC can evaluate the effects of therapy. Another group o
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