Influence of ABO Blood Group on von Willebrand Factor Antigen Level in Normal Individuals: A Cross-Sectional Study from
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Influence of ABO Blood Group on von Willebrand Factor Antigen Level in Normal Individuals: A Cross-Sectional Study from Southern India Pagadalu Lokababu Ambika1 • Rakhee Kar1 Rajendra Gurunath Kulkarni2
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Debdatta Basu1,2
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Received: 6 July 2020 / Accepted: 6 October 2020 Ó Indian Society of Hematology and Blood Transfusion 2020
Sir, The ABO blood group type has a potential influence on hemostasis due to its effect on von Willebrand factor (VWF) and thereby on factor VIII plasma levels. ABO blood group genotyping has shown that O1O1 subjects have the lowest VWF levels, and non-O group individuals heterozygous for the O1 allele have significantly lower VWF levels than AA, AB, or BB subjects [1]. Studies on the effect of ABO blood group on the plasma levels of VWF have been carried out in different ethnicities [2, 3] but there is paucity of information in this regard from India. The aim of this study was to determine the influence of ABO blood group on plasma VWF:Ag level in normal South Indian population. This was a cross-sectional analytical study over a 3-year period (May 2015 to May 2018) conducted in the Departments of Pathology and Transfusion Medicine in a tertiary care hospital in Southern India. Three hundred ninety four fit and healthy apheresis donors selected as per statutory drugs and cosmetic rules, and other Govt. of India guidelines were enrolled. After informed consent, blood was collected from each subject; 2 ml in EDTA for blood counts and forward ABO blood grouping and Rh typing (cell grouping); 3 ml in plain vial for the confirmation of ABO blood group by serum (reverse) grouping, antibody
& Rakhee Kar [email protected] 1
Department of Pathology, Jawaharlal Institute of Postgraduate Medical Education and Research, Puducherry 605006, India
2
Department of Transfusion Medicine, Jawaharlal Institute of Postgraduate Medical Education and Research, Puducherry 605006, India
and transfusion transmitted infection screen; and additional 2 ml in citrate for the estimation of VWF:Ag. Standard tube technique was done using Anti- A, Anti-B, Anti- H and Anti-D monoclonal antisera (Tulip Diagnostics, India). For reverse blood grouping freshly prepared 5% known A, B, O blood group pooled red cells were used. The samples for the determination of VWF:Ag were centrifuged at 1500 g for 15 min and plasma was stored at -40˚C until assayed. The maximum duration of sample storage was 6–8 months. Estimation of VWF:Ag was carried out in batches using Enzyme Linked Immunosorbent Assay (ELISA). The kits (Raybiotech Life, Georgia, United States) were stored as per the manufacturer’s recommendation. Lyophilized standards supplied with kit were used for making the standard curve. The unit of plasma VWF:Ag level as per the kit insert was ng/ml. One way analysis of variance (ANOVA) was used to compare the mean VWF:Ag levels between the various ABO blood group phenotypes. The age of donors ranged from 18–55 years with mean of 27.4 ± 7.4 years and all were male. The distribution of ABO blood groups wa
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