Influence of organic solvents in the extraction and purification of torularhodin from Sporobolomyces ruberrimus
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ORIGINAL RESEARCH PAPER
Influence of organic solvents in the extraction and purification of torularhodin from Sporobolomyces ruberrimus Karen Yuri Feitosa Kanno . Susan Grace Karp . Cristine Rodrigues . Valcineide Oliveira de Andrade Tanobe . Carlos Ricardo Soccol . Ligia Alves da Costa Cardoso
Received: 11 March 2020 / Accepted: 5 October 2020 Ó Springer Nature B.V. 2020
Abstract Objective This work aimed at evaluating the influence of organic solvents and stationary phases in the extraction with glass beads and chromatographic purification of carotenoids, especially torularhodin, from Sporobolomyces ruberrimus. Results The combinations of acetone:hexane (1:1 v/v) and acetone:ethyl ether (1:1 v/v) yielded 171.74 and 172.19 lg of total carotenoids.g of cells-1, respectively. The first blend resulted in the highest percent of cell lysis of 57.4%. Among different proportions of acetone:hexane, the 9:1 v/v mixture showed a significant difference (p\0.05), resulting in a recovery of total carotenoids of 221.88 lg.g of cells-1. The purification of carotenoids was made by preparative chromatography and the yield of the silicacontaining stationary phase was higher (24 lg torularhodin.g cells-1). The analyses of the purified fractions in thin layer chromatography and high performance liquid chromatography indicated that
K. Y. F. Kanno L. A. da Costa Cardoso (&) Doctorate and Master’s Degree Graduation Program in Industrial Biotechnology, Universidade Positivo, Curitiba, PR, Brazil e-mail: [email protected] S. G. Karp C. Rodrigues V. O. de Andrade Tanobe C. R. Soccol Graduate Program in Bioprocess Engineering and Biotechnology, Universidade Federal do Parana´, Curitiba, PR, Brazil
the purification of carotenoids, especially of torularhodin, was successfully performed. Conclusions The combination of polar (acetone) and non-polar solvents (hexane) and the use of silica as stationary phase was efficient to recover and purify torularhodin from the intracellular pigments of Sporobolomyces ruberrimus. Keywords Carotenoids Yeast Cell rupture Separation Chromatography
Introduction Carotenoids are a class of natural pigments found in several microorganisms, such as microalgae, filamentous fungi, bacteria and yeasts that synthesize these molecules as secondary metabolites (Cardoso et al. 2017). Rhodotorula sp., Rhodosporidium sp. and Sporobolomyces sp. produce carotenoids such as bcarotene, c-carotene, torulene and torularhodin (Buzzini et al. 2007; Tka´cova´ et al. 2015; Zhang et al. 2016). They exhibit antioxidant activity that can prevent cardiovascular diseases, cancer, diabetes and Alzheimer’s disease (Milani et al. 2017; Grudzinski et al. 2018; Yamagata 2017; Roohbakhsh et al. 2017; Olasehinde et al. 2017), so there is industrial interest in the use of these natural biomolecules as an
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Biotechnol Lett
alternative to the synthetic additives (Petruk et al. 2019). Yeast carotenoids are located in the interior of lipid micelles (Cheng et al. 2016), so
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