Influence of Phleum pratense pollen grains rupture on lipids extraction
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ORIGINAL PAPER
Influence of Phleum pratense pollen grains rupture on lipids extraction Jinane Farah . Marie Choe¨l . Patricia de Nadaı¨ . Joanne Balsamelli . Sylvie Gosselin . Nicolas Visez
Received: 29 March 2020 / Accepted: 12 September 2020 Springer Nature B.V. 2020
Abstract In this work, we completed the first identification and quantification of internal lipids for allergenic Phleum pratense pollen grains (PGs). We have extracted PGs with aqueous solvent under different extraction procedures and observed release of extra amount of unsaturated fatty acids (a-linolenic and linoleic acids) when PGs were broken during extraction. We showed that rupture of PGs is necessary for the release of higher quantity of bioactive compounds. Organic solvent extraction of crushed PGs confirmed the predominance of unsaturated fatty acids in the inner parts of PGs as well as a different distribution of external and internal lipids. The total mass of extractible matter by organic solvent was determined at 22 ± 1 mg g-1 for intact pollen and at 48 ± 1 mg g-1 for broken pollen. This finding brings new insight into the physical degradation of PGs by air pollutants during atmospheric transport. We
hypothesize that the presence of bioactive lipids in pollen cytoplasm gives further importance to asthma triggered by cracked or broken allergenic PGs. The rupturing of PGs in the atmosphere may expose the population to the risk of inhaling micrometer-sized aerosols (cytoplasmic granules) containing not only allergens but also bioactive lipids.
J. Farah S. Gosselin N. Visez CNRS, UMR 8522 - PC2A - Physicochimie des Processus de Combustion et de l’Atmosphe`re, Univ. Lille, 59000 Lille, France
P. de Nadaı¨ J. Balsamelli CNRS, Inserm, CHU Lille, Institut Pasteur de Lille, U1019 - UMR 8204 - CIIL - Center for Infection and Immunity of Lille, Univ. Lille, 59000 Lille, France
J. Farah Faculte´ de sante´ publique section III, Laboratoire des Sciences de l’Eau et de l’Environnement (L.S.E.E), Universite´ Libanaise, Tripoli, Lebanon
N. Visez Association pour la Pre´vention de la Pollution Atmosphe´rique, Parc Eurasante´, 235 avenue de la Recherche, 59120 Loos, France
M. Choe¨l CNRS, UMR 8516 - LASIRE - Laboratoire de Spectroscopie pour les Interactions, la Re´activite´ et l’Environnement, Univ. Lille, 59000 Lille, France
N. Visez (&) Universite´ de Lille, Baˆtiment C11, Cite´ Scientifique, 59655 Villeneuve d’Ascq, France e-mail: [email protected]
Keywords Pollen allergy Lipidomic Timothy grass Allergenic pollen Cytoplasmic granules
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Aerobiologia
1 Introduction Pollen grains (PGs) from grasses are an important trigger of asthmatic attacks (D’Amato et al. 2008, 2016; Idrose et al. 2019; Knox 1993; Newson et al. 1997; Porcel Carren˜o et al. 2019; Simunovic et al. 2020). Grass PGs are considered too large (25–60 lm) to reach the lower respiratory system where asthmatic responses occur (Wilson et al. 1973). Asthma attacks induced by grass PGs have been linked to poll
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