Influence of rehydration on transcriptome during resuscitation of desiccated Pseudomonas putida KT2440
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López-Lara et al. Annals of Microbiology (2020) 70:54 https://doi.org/10.1186/s13213-020-01596-3
ORIGINAL ARTICLE
Open Access
Influence of rehydration on transcriptome during resuscitation of desiccated Pseudomonas putida KT2440 Lilia I. López-Lara1, Laura A. Pazos-Rojas1, Lesther E. López-Cruz1, Yolanda E. Morales-García1,2, Verónica Quintero-Hernández1,3, Jesús de la Torre4, Pieter van Dillewijn4, Jesús Muñoz-Rojas1,5* and Antonino Baez1,5*
Abstract Purpose: Pseudomonas putida KT2440 is a desiccation-sensitive bacterium that loses culturability after 15 days of air desiccation. We have previously shown that P. putida KT2440 can develop a viable but nonculturable (VBNC) state after being exposed to desiccation stress and eventually recover when desiccated cells are rehydrated for at least 24 h. Methods: To determine which genes of transport, oxidation-reduction, and transcription processes could be involved in the return of P. putida KT2440 to the culturable state, a transcriptome analysis was carried out comparing the gene expression of non-desiccated samples with samples subjected to desiccation followed by 20 min of rehydration or desiccation followed by 24 h of rehydration. Results: Desiccation stress triggered a VBNC state of P. putida. The major response was detected after 24 h of rehydration with 148 upregulated and 42 downregulated genes. During the VBNC state, P. putida activated transmembrane transport processes like that of siderophores through a TonB-dependent transporter and putative polyhydric alcohol transport systems. Prolonged rehydration with distilled water resuscitated P. putida KT2440 cells activating the catabolism of phenylalanine/tyrosine to provide energy and carbon for ubiquinone biosynthesis while maintaining a reduced protein synthesis. On the other hand, the interruption of the TonB-dependent receptor gene (PP_1446) increased desiccation survival of the mutant strain. Conclusion: The activation of the iron transport system (TonB-dependent siderophore receptor) and alcohol transport can be helping the VBNC state of P. putida. Activation of catabolism of phenylalanine/tyrosine and reduced protein synthesis was needed for resuscitation from the VBNC state. Keywords: TonB-dependent receptor, Resuscitation, Survival, VBNC
Background Beneficial interactions between plants and bacteria greatly favor plant growth and its protection against diseases and pests (Kloepper et al. 1989; Hayat et al. 2010). A successful interaction not only relies on the capabilities of the beneficial bacteria but also on abiotic factors such as temperature, pH, salinity, and water availability that can * Correspondence: [email protected]; [email protected] 1 Centro de Investigaciones en Ciencias Microbiológicas, Instituto de Ciencias, Benemérita Universidad Autónoma de Puebla, Puebla, México Full list of author information is available at the end of the article
disrupt the bacterial-plant interaction (Molina-Romero et al. 2017; Enebe and Babalola 2018). Water limiting conditions affect the viabil
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