Inhibition of berberine hydrochloride on Candida albicans biofilm formation
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ORIGINAL RESEARCH PAPER
Inhibition of berberine hydrochloride on Candida albicans biofilm formation Xiaoxue Huang . Mingyue Zheng . Yuling Yi . Anamica Patel . Zhen Song . Yan Li
Received: 29 December 2019 / Accepted: 5 June 2020 Ó Springer Nature B.V. 2020
Abstract Objective This study aimed to investigate the inhibitory effect of berberine hydrochloride (BH) on Candida albicans (C.albicans) ATCC10231 biofilm formation. Results This paper found a positive correlation between the concentration of BH and its inhibitory effect on the cellular activity of early biofilms because we found that 128 and 32 lg/mL BH significantly inhibited biofilm formation (P \ 0.05). BH significantly inhibited the cellular activity in early biofilms, destroyed the microscopic morphology of C.albicans
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s10529-020-02938-6) contains supplementary material, which is available to authorized users. X. Huang Y. Yi Z. Song Y. Li (&) College of Medical Technology, Chengdu University of Traditional Chinese Medicine, Sichuan 611137, China e-mail: [email protected] M. Zheng School of Health and Rehabilitation, Chengdu University of Traditional Chinese Medicine, Sichuan 611137, China M. Zheng Adelaide Medical School, University of Adelaide, Adelaide, SA 5005, Australia A. Patel Observatory Evidence Service, Public Health Wales, Cardiff CF10 4BZ, UK
and reduced the thickness of the biofilm. Both 128 and 32 lg/mL concentration solutions of BH significantly inhibited biofilm formation (P \ 0.05). We found that the inhibitory effect of BH solution was positively correlated with its concentration and 128 lg/mL BH was better than 4 lg/mL fluconazole. Additionally, the results of RT-PCR indicated that 128 and 32 lg/ mL BH inhibited the expression of EFG1, HWP1, ECE1, and ALS1 (P \ 0.05). Conclusion The efficacy of BH in inhibiting the formation of C.albicans biofilm by killing the cells in the biofilm and destroying its structure; and the mechanism may be to down-regulate the expression of EFG1, HWP1, ECE1, and ALS1 in hyphae formation, thereby, retarding the morphological transformation of C. albicans. Keyword Berberine hydrochloride Biofilm formation Candida albicans Natural antifungal agents
Introduction Candida albicans (C.albicans) is defined as a symbiotic body on the mucosal surface of most healthy people, but it can cause life-threatening systemic infections in immunocompromised individuals. C.albicans infection is the fourth most common type of
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Biotechnol Lett
hospital bloodstream infection (Gulati and Nobile 2016). The formation of biofilm by C.albicans, increases its drug resistance and virulence (Xie et al. 2020). The development of new natural antifungal agents could effectively control C.albicans infection regarding efficient and safe derivatives. BH is an isoflavone alkaloid isolated from berberine, an active ingredient found in Coptisa. BH has a variety of activities against
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