Insight into Evolution and Conservation Patterns of B1-Subfamily Members of GPCR
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Insight into Evolution and Conservation Patterns of B1‑Subfamily Members of GPCR Chiranjib Chakraborty1,2 · Ashish Ranjan Sharma2 · Garima Sharma3 · Manojit Bhattacharya2 · Sang‑Soo Lee2 Accepted: 30 January 2020 © Springer Nature B.V. 2020
Abstract The diverse, evolutionary architectures of proteins can be regarded as molecular fossils, tracing a historical path that marks important milestones across life. The B1-subfamily of GPCRs (G-protein-coupled receptors) are medically significant proteins that comprise 15 transmembrane receptor proteins in Homo sapiens. These proteins control the intracellular concentration of cyclic AMP as well as various vital processes in the body. However, little is known about the evolutionary correlation and conservational blueprint of this GPCR subfamily. We performed a comprehensive analysis to understand the evolutionary architecture among 13 members of the B1-subfamily. Multiple sequence alignment analysis exhibited six multiple sequence aligned blocks and five highly aligned blocks. Molecular phylogenetics indicated that CRHR1 and CRHR2 share a typical ancestral relationship and are siblings in 100% bootstrap replications with a total of 24 nodes observed in the cladogram. CRHR2 has the maximum number of extremely conserved amino acids followed by ADCYAP1R1. The longest continuous number sequence logos (74) were found between sequence location 349 and 423, and consequently, the maximum and minimum logo height recorded was 3.6 bits and 0.18 bits, respectively. Finally, to understand the model and pattern of evolutionary relatedness, the conservation blueprint, and the diversification among the members of a protein family, GPCR distribution from several species throughout the animal kingdom was analysed. Together, the study provides an evolutionary insight and offers a rapid method to explore the potential of depicting the evolutionary relationship, conservation blueprint, and diversification among the B1-subfamily of GPCRs using bioinformatics, algorithm analysis, and mathematical models. Keywords Secretin receptor · B1-subfamily · In silico · Evolutionary relationship
Introduction Chiranjib Chakraborty and Ashish Ranjan Sharma have contributed equally to this work. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s10989-020-10043-5) contains supplementary material, which is available to authorized users. * Chiranjib Chakraborty [email protected] * Sang‑Soo Lee [email protected] 1
Adamas University, North, 24 Parganas, Kolkata 700126, West Bengal, India
2
Institute for Skeletal Aging & Orthopedic Surgery, Chuncheon Sacred Heart Hospital, Hallym University, Chuncheon 24252, Republic of Korea
3
Neuropsychopharmacology and Toxicology Program, College of Pharmacy, Kangwon National University, Chuncheon 24341, Republic of Korea
The name “protein family” was proposed by Dayhoff in the 1960s to describe proteins with similar structure and/or function, which have followed the evolution process from a common ancestor
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