Integrated analysis of miRNA and mRNA expression profiles in testes of Duroc and Meishan boars
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RESEARCH ARTICLE
Open Access
Integrated analysis of miRNA and mRNA expression profiles in testes of Duroc and Meishan boars Haisheng Ding1,2†, Min Liu1†, Changfan Zhou1, Xiangbin You1, Tao Su1, Youbing Yang3 and Dequan Xu1*
Abstract Background: MicroRNAs (miRNAs) are small non-coding RNAs playing vital roles in regulating posttranscriptional gene expression. Elucidating the expression regulation of miRNAs underlying pig testis development will contribute to a better understanding of boar fertility and spermatogenesis. Results: In this study, miRNA expression profile was investigated in testes of Duroc and Meishan boars at 20, 75, and 270 days of age by high-throughput sequencing. Forty-five differentially expressed miRNAs were identified from testes of Duroc and Meishan boars before and after puberty. Integrated analysis of miRNA and mRNA profiles predicted many miRNA-mRNA pairs. Gene ontology and biological pathway analyses revealed that predicted target genes of ssc-mir-4235p, ssc-mir-34c, ssc-mir-107, ssc-mir-196b-5p, ssc-mir-92a, ssc-mir-320, ssc-mir-10a-5p, and ssc-mir-181b were involved in sexual reproduction, male gamete generation, and spermatogenesis, and GnRH, Wnt, and MAPK signaling pathway. Four significantly differentially expressed miRNAs and their predicted target genes were validated by quantitative real-time polymerase chain reaction, and phospholipase C beta 1 (PLCβ1) gene was verified to be a target of ssc-mir-423-5p. Conclusions: This study provides an insight into the functional roles of miRNAs in testis development and spermatogenesis and offers useful resources for understanding differences in sexual function development caused by the change in miRNAs expression between Duroc and Meishan boars. Keywords: Meishan boar, Duroc boar, Testis, Sexual development, miRNA, Integrating analysis
Background Testis is an important male reproductive and endocrine organ which is a critical tissue for spermatogenesis. Spermatogenesis is a complicated process including mitotic cell division, meiosis, and the process of spermiogenesis [1]. Spermatogenesis is strictly regulated by the expression of stage-specific genes in testis at both transcription and post-transcription levels [2]. Identifying key * Correspondence: [email protected] † Haisheng Ding and Min Liu contributed equally to this work. 1 Key Laboratory of Swine Genetics and Breeding of Ministry of Agriculture and Rural Affairs, and Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan 430070, People’s Republic of China Full list of author information is available at the end of the article
regulators in testis development and spermatogenesis will provide valuable insights into the mechanism of sexual function development [3]. As a class of endogenous small (~ 22 nucleotides) noncoding RNAs, miRNAs mediate post-transcriptional gene expression in animals and fine-tune the expression of approximately 30% of all mammalian protein-coding genes [4–6]. In addition, miRNAs regu
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