miRNA Expression Profiles in Cerebrospinal Fluid and Blood of Patients with Acute Ischemic Stroke
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ORIGINAL ARTICLE
miRNA Expression Profiles in Cerebrospinal Fluid and Blood of Patients with Acute Ischemic Stroke Sofie Sølvsten Sørensen & Ann-Britt Nygaard & Ming-Yuan Nielsen & Kai Jensen & Thomas Christensen
Received: 3 April 2014 / Revised: 25 June 2014 / Accepted: 21 July 2014 # Springer Science+Business Media New York 2014
Abstract The aims of the study were (1) to determine whether miRNAs (microRNAs) can be detected in the cerebrospinal fluid (CSF) and blood of patients with ischemic stroke and (2) to compare these miRNA profiles with corresponding profiles from other neurological patients to address whether the miRNA profiles of CSF or blood have potential usefulness as diagnostic biomarkers of ischemic stroke. CSF from patients with acute ischemic stroke (n=10) and patients with other neurological diseases (n=10) was collected by lumbar puncture. Blood samples were taken immediately after. Expression profiles in the cell-free fractions of CSF and blood were analyzed by a microarray technique (miRCURY LNA™ microRNA Array, Exiqon A/S, Denmark) using a quantitative PCR (qPCR) platform containing 378 miRNA primers. In total, 183 different miRNAs were detected in the CSF, of which two miRNAs (let-7c and miR-221-3p) were found upregulated in relation to stroke. In the blood, 287 different miRNAs were detected of which two miRNAs (miR-151a-3p and miR-140-5p) were found upregulated and one miRNA (miR-18b-5p) was found downregulated in the stroke group. Some miRNAs occurred exclusively in the CSF including miR-523-3p which was detected in 50 % of the stroke Electronic supplementary material The online version of this article (doi:10.1007/s12975-014-0364-8) contains supplementary material, which is available to authorized users. S. S. Sørensen (*) : K. Jensen : T. Christensen Department of Neurology, Nordsjællands Hospital, Copenhagen University, Dyrehavevej 29, 3400 Hillerød, Denmark e-mail: [email protected] A.
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