Interference with circBC048201 inhibits the proliferation, migration, and invasion of bladder cancer cells through the m
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Interference with circBC048201 inhibits the proliferation, migration, and invasion of bladder cancer cells through the miR‑1184/ITGA3 axis Peng Jiang1 · Yi Zhu1 · Zhijie Xu1 · Xiaoyi Chen1 · Liping Xie1 Received: 26 April 2020 / Accepted: 11 July 2020 © Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract The abnormal expression of circular RNA (circRNA) is bound up with the progress of various human cancers. This study aimed to reveal the potential role and mechanism of circBC048201 in the proliferation, migration, and invasion of bladder cancer cells. Quantitative real-time PCR was performed to detect the expression of circBC048201. Cell Counting Kit-8, colony formation, and transwell migration and invasion assays were used to confirm the in vitro functions of circBC048201. Western blot, RNA pull-down, and dual-luciferase reporter gene experiments were performed to study the potential mechanism. circBC048201 was abnormally highly expressed in bladder cancer tissues and cells, and the interference with circBC048201 inhibited bladder cancer cell proliferation, migration, and invasion. From the potential mechanism analysis, our data suggested that circBC048201 and miR-1184, miR-1184 and ITGA3 could bind to each other, and the interference with circBC048201 repressed bladder cancer cell proliferation, migration, and invasion through the miR-1184/ITGA3 axis. In summary, our results showed that circBC048201 was abnormally highly expressed in bladder cancer tissues and cells, and the interference with circBC048201 inhibited the proliferation, migration, and invasion of bladder cancer cells through the miR-1184/ITGA3 axis. Keywords circBC048201 · miR-1184 · ITGA3 · Proliferation · Migration and invasion · Bladder cancer
Introduction Bladder cancer is one of the malignant tumors in the world, which has high morbidity and high mortality [1]. Unfortunately, there is still a lack of effective treatment for bladder cancer in the clinic. As reported, the proliferation, migration, and invasion of bladder cancer are important causes of the recurrence and death of patients with bladder cancer [2, 3]. Therefore, elucidating the underlying mechanism of bladder cancer cell proliferation, migration, and invasion has the potential to fight bladder cancer. Circular RNA (circRNA) is an endogenous non-coding RNA composed of a closed circular structure [4], and most Electronic supplementary material The online version of this article (https://doi.org/10.1007/s11010-020-03835-2) contains supplementary material, which is available to authorized users. * Liping Xie [email protected] 1
Department of Urology, The First Affiliated Hospital, College of Medicine, Zhejiang University, No. 79, Qingchun Road, Hangzhou 310003, Zhejiang, China
of the biosynthesis of circRNA is derived from reverse splicing events [5]. Several previous studies have shown that circRNAs have multiple functions; mainly they act as the sponges of miRNAs and regulate splicing [6]. Growing body studies indicate that circRNAs play important
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