miR-573 suppresses pancreatic cancer cell proliferation, migration, and invasion through targeting TSPAN1
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ORIGINAL ARTICLE
miR-573 suppresses pancreatic cancer cell proliferation, migration, and invasion through targeting TSPAN1 Lei Wang1 · Peng Gao2 · Ping Yuan3 · Pengcheng Zhou4 · Haowen Fan5 · Xida Lin5 · Xiaoyu Yuan6 · Mingyan Zhu1 · Xiangjun Fan1 · Yuhua Lu1 · Zhiwei Wang1 Received: 5 August 2020 / Accepted: 30 November 2020 © Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract Purpose To explore whether miR-573 can suppress pancreatic cancer cell proliferation, migration, and invasion by targeting TSPAN1. Methods The expression of miR-573 and TSPAN1 in pancreatic cancer tissues and cells lines was analyzed using RT-qPCR. The human pancreatic cancer cell line PANC-1 was transfected with miR-573 mimic, pcDNA3.1-TSPAN1, or genOFFTM st-h-TSPAN1. The effects of miR-573 and TSPAN1 on cell proliferation, colony formation, migration, and invasion were analyzed by CCK-8, colony formation, transwell migration, and invasion assay, respectively. Target genes of miR-573 were screened using bioinformatics tools and confirmed by dual-luciferase reporter assay and real-time PCR. The effects of miR-573 in vivo were observed using tumor xenografts. Results We found that miR-573 is downregulated and TSPAN1 is upregulated in pancreatic cancer tissues and cells lines. Function assays demonstrated that overexpression of miR-573 inhibited cell proliferation, colony formation, migration, and invasion of pancreatic cancer cells, as well as suppressing tumor growth in vivo. Target genes of miR-573 were predicted using bioinformatics tools and confirmed by dual-luciferase reporter assay and RT-qPCR or western blotting. Downregulation of TSPAN1 also inhibited cell proliferation, colony formation, migration, and invasion of pancreatic cancer cells. Furthermore, overexpression of TSPAN1 attenuated miR-573-induced inhibition of pancreatic cancer cell proliferation and migration. Peng Gao and Lei Wang contributed equally to this work. Lei Wang [email protected] Peng Gao [email protected] Ping Yuan [email protected] Pengcheng Zhou [email protected]
Zhiwei Wang
[email protected] 1
Department of General Surgery, Affiliated Hospital of Nantong University, Nantong University, 226001 Nantong, China
2
Department of General Surgery, Nantong Hospital of Traditional Chinese Medicine, Nantong University, 226001 Nantong, China
3
Department of Hand Surgery, Affiliated Hospital of Nantong University, Nantong University, 226001 Nantong, China
4
Department of Emergency Surgery, Affiliated Hospital of Nantong University, Nantong University, 226001 Nantong, China
5
Department of surgical comprehensive laboratory, Affiliated Hospital of Nantong University, Nantong University, 226001 Nantong, China
6
Department of Emergency Internal Medicine, Affiliated Hospital of Nantong University, Nantong University, 226001 Nantong, China
Haowen Fan [email protected] Xida Lin [email protected] Xiaoyu Yuan [email protected] Mingyan Zhu [email protected] Xiangjun Fan [email protected] Yuhua Lu
[email protected]
K
Strahlenther Onkol
Conclusion
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