Intestinal tract and parenteral multi-organ sequential pathological injury caused by necrotizing enterocolitis
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RESEARCH ARTICLE
Open Access
Intestinal tract and parenteral multi-organ sequential pathological injury caused by necrotizing enterocolitis Fu-Sheng Wang1, Meng-Lu Yu1, Wei-Zhong Li2, Kai Hong3, Chen-Bin Xu1 and Guang-Huan Wang1*
Abstract Background: To explore the relationship between the pathological changes of the colon, terminal ileum, lung, liver and kidney, and the changes of Bax, PCNA and PAF in a rat model of NEC. Methods: One hundred and forty neonatal SD rats were randomly divided into NEC group and control group (70 in each group). NEC group was given hypoxia, cold stimulation and artificial feeding twice a day for 3 consecutive days. The control group was only fed normally. After modeling, From the 1st day to the 7th day, 10 rats were sampled in each group for pathological examination of colon, terminal ileum, lung, liver and kidney tissue. The levels of Bax, PCNA and PAF were investigated by immunohistochemistry. Results: Compared with the normal group, in the NEC group, on the 1st day, the colon, terminal ileum, lung, liver and kidney showed inflammatory damage. On the 5th day, the inflammatory injury was reduced. The inflammation disappeared on the 7th day. There were differences in the time of apoptosis in the intestine. In the intestine, the proliferation of PCNA was weak at first and then strong. Bax in liver and kidney showed marked apoptosis and apoptosis time increased in the lung. The expression of PCNA increased in lung, liver and kidney, and the expression of PAF increased in lung and liver. Conclusions: NEC can lead to secondary injury of different degrees in colon, terminal ileum, lung, liver and kidney, and the degree and time of injury and repair were different. In general, organ repair played a leading role on the 4th day after modeling. Keywords: Necrotizing enterocolitis, Bcl2-related X gene, Platelet-activating factor, Proliferating cell nuclear antigen
Background Neonatal NEC is a serious life-threatening gastrointestinal emergency in the neonatal stage. It is one of the most destructive diseases in neonates [1]. Research on the pathogenesis and specific treatment of NEC has become an essential subject in pediatrics. At present, the study of NEC mainly focuses on the aetiology and pathogenesis, as well as the protection and prevention of * Correspondence: [email protected] 1 Department of Pediatric Surgery, The Second Affiliated Hospital of Shantou University Medical College, Shantou 515041, China Full list of author information is available at the end of the article
injured intestinal organs. However, there is a lack of systematic research and understanding of the pathological and functional changes of various organs in the whole body after the occurrence of NEC. We established an animal model of NEC to study pathological changes of the colon, terminal ileum, lung, liver and kidney after induced NEC, and then detected the expression of Bax, PCNA and PAF in these organs [2]. Bax is a vital apoptosis promoting gene, and its rise indicates that the cell starts the apoptosis proce
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