Intracellular Ca 2+ and related proteins in patients with oral lichen planus
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ORIGINAL ARTICLE
Intracellular Ca2+ and related proteins in patients with oral lichen planus Jiang-Min Ma1,3 • Ran Wang1,4 • Juan-Yong Xu1,2 • Yuan Fan1,2
Ó Springer Science+Business Media New York 2015
Abstract Oral lichen planus (OLP) is suggested to be a T cell-mediated chronic inflammatory oral mucosal disease. Gene expressed in the oligodendrocyte lineage–myelin basic proteins (Golli-MBP) and stromal interaction molecule 1 (STIM1) are important in the activation and function of T lymphocytes. This study aimed to analyze and compare the expression of Golli-MBP and STIM1 between OLP patients and healthy controls and to analyze the level of intracellular Ca2?, which is involved in lymphocyte activation. The Ca2? fluorescent probe, Fluo-3/AM, was used to test the level of intracellular Ca2? in patients with OLP and healthy controls peripheral blood lymphocytes. Golli-MBP and STIM1 mRNA and protein levels were analyzed using quantitative real-time PCR and Western blot, respectively. Following lymphocyte activation, the intracellular Ca2? in OLP patients was markedly lower than that in the control group (P \ 0.001). In OLP patients, the expression of Golli-MBP mRNA and protein was significantly upregulated compared
Jiang-Min Ma and Ran Wang have contributed equally to this work. & Yuan Fan [email protected] 1
Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, 136#, Hanzhong Road, Nanjing 210029, Jiangsu, People’s Republic of China
2
Department of Oral Medicine, Affiliated Hospital of Stomatology, Nanjing Medical University, 136#, Hanzhong Road, Nanjing 210029, Jiangsu, People’s Republic of China
3
Present Address: Department of Stomatology, Kunshan Hospital of Traditional Chinese Medicine, 189#, Chaoyang Road, Kunshan 215300, Jiangsu, People’s Republic of China
4
Present Address: Department of Stomatology, Nanjing Red Cross Hospital, 242#, Baixia Road, Nanjing 210000, Jiangsu, People’s Republic of China
to those of the control group (P \ 0.001). Similarly, OLP patients showed markedly upregulated levels of STIM1 mRNA expression (P \ 0.01) and protein compared to healthy controls. The intracellular Ca2? of OLP patients was markedly lower than that of healthy controls. This evidence may indicate that Ca2? signaling pathways in OLP patients are abnormal. The overexpression of Golli-MBP and STIM1 may play a role in the pathogenesis of OLP. Keywords Oral lichen planus Genes of the oligodendrocyte lineage Stromal interaction molecule 1 Intracelluar calcium
Introduction Oral lichen planus (OLP) is a T cell-mediated disorder of the oral mucosa that is estimated to occur in 0.1–4 % of the population [1]. The typical oral lesions are striations (striae of Wickham), papules, plaques, mucosal atrophy, erosions (shallow ulcers) or blisters affecting the buccal mucosa, tongue and gingiva [1]. In clinical practice, they are often divided into two groups: non-erosion type and erosion type. The main histological features of OLP include liquefaction degeneration of basal epithelial cells, lymp
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