Isolation and characteristics of the human rotavirus isolate CY2017
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ORIGINAL ARTICLE
Isolation and characteristics of the human rotavirus isolate CY2017 Yan Song1
•
Wei Liu1
Received: 12 December 2019 / Accepted: 26 May 2020 Ó The Author(s) 2020
Abstract Our study aimed to determine whether a virus from fecal samples is human rotavirus (HRV), the main pathogen that causes autumn diarrhea. Specimens were obtained from the feces from children with autumn diarrhea treated at Chuiyangliu Hospital and used to infect MA104 cells, subcultured and observed by electron microscopy. RNA was extracted, cDNA was synthesized by reverse transcription, and plaque formation and hemagglutination were assessed. The cytopathic effect (CPE) were associated with the fourth passage in subculture. CPE extracts were examined by electron microscopy, which allowed us to observe the shape of HRV particles. Amplification of the VP4 gene from HRV was used to identify the viruses as group A rotavirus. The virus causes red blood cell aggregation. The virus isolate was designated as HRV CY2017.
cases of intestinal viral infection [4, 7]. At present, group A HRV is the world’s most common HRV strain. Indeed, statistics from the World Health Organization indicate that HRV infection can explain approximately 600,000 deaths among children under 5 years globally. Group A HRVs are the most common etiologic agents of severe diarrhea in infants and young children worldwide and are responsible for 2 million hospitalizations globally [13]. In China, HRV infection causes 40–60% of cases of autumn and winter diarrhea in infants and young children [7], and studies have demonstrated that improving sanitation has limited effectiveness in preventing HRV-induced diarrhea [4, 7].
Material and methods Specimen collection
Keywords Rotavirus Isolation and identification Characteristics research
Introduction Diarrhea is common in children. Autumn diarrhea, a subcategory of this disease, is mostly caused by human rotavirus (HRV), which belongs to the genus Rotavirus and the family Reoviridae [7, 13]. Epidemiological investigations have shown that HRV is the main pathogen that causes nonbacterial gastroenteritis, accounting for [ 50% of all & Yan Song [email protected] 1
Affiliated Chuiyangliu Hospital of Tsinghua University, Beijing 100022, China
Fecal specimens were obtained from children diagnosed with autumn diarrhea at Chuiyangliu Hospital. The children developed acute inflammatory digestive tract symptoms, such as fever, vomiting, and diarrhea. MA104 cells and red blood cells (RBCs) A transformed Monkey fetal kidney cell line (MA104) was purchased from the China Center for Type Culture Collection of Wuhan University. The cells were subcultured in Dulbecco’s modified Eagle’s medium (DMEM) containing 12% fetal bovine serum. Healthy human RBCs from each of the four human ABO blood groups were obtained from the blood bank at the hospital. RBCs from albino mice, rabbits, and dogs were obtained from sterile animals at the Key Laboratory of Infection and Immunity of the Chinese Academy of Sciences.
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