Isolation and characterization of 18 microsatellites for Aconitum vilmorinianum Kom. (Ranunculaceae) using next-generati
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MICROSATELLITE LETTERS
Isolation and characterization of 18 microsatellites for Aconitum vilmorinianum Kom. (Ranunculaceae) using next-generation sequencing technology Jun He • Zhi-Rong Zhang • Jun-Bo Yang Hong Wang • Jing Meng
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Received: 8 January 2015 / Accepted: 4 February 2015 Ó Springer Science+Business Media Dordrecht 2015
Abstract Aconitum vilmorinianum is a very important Chinese traditional medicinal plant, which is one of the main raw materials in Yunnan Baiyao as an analgesic and anti-inflammatory agent. Recently, it has been threatened by overexploitation and human disturbances. Genetic background of this species is essential for the efficient conservation and rational utilization. Therefore, a total of 18 novel microsatellite markers were developed for A. vilmorinianum using next-generation sequencing technology. The number of alleles per locus ranged from 1 to 5, with a mean of 2.560, and the observed and expected heterozygosities ranged from 0 to 1.000 and from 0 to 0.733, respectively. These polymorphic microsatellite loci will be especially useful for genetics studies of this important medicinal plant. Keywords Aconitum vilmorinianum Microsatellite markers Genetics Medicinal plant
Electronic supplementary material The online version of this article (doi:10.1007/s12686-015-0432-8) contains supplementary material, which is available to authorized users. J. He Z.-R. Zhang J.-B. Yang Germplasm Bank of Wild Species, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650204, China H. Wang Key Laboratory of Biodiversity and Biogeography, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650204, China J. Meng (&) College of Horticulture and Landscape, Yunnan Agricultural University, Kunming 650201, China e-mail: [email protected]
Aconitum L. (Ranunculaceae) is a large genus with about 300 species and mainly distributes in the temperate regions of the Northern Hemisphere. About 76 Aconitum species in China are poisonous and have been used as medicinal plants mainly for treatment of rheumatoid arthritis and various types of pains (Xiao et al. 2006). These species have similar organoleptic characteristics with different toxicities and chemical constituents. Aconitum vilmorinianum Kom. is a climbing perennial herb endemic to China, and wildly distributed in the montane shrubland of Yunnan, Sichuan and Guizhou Provinces. The tuberous mother roots of A. vilmorinianum (‘Huangcaowu’, used by Chinese druggists), is one of the main raw materials in Yunnan Baiyao, which is Chinese Class-1 protected traditional medicine and has been honored as ‘trauma panacea’ for more than a century (Li et al. 2009). Aconitum vilmorinianum has been used as a popular analgesic and anti-inflammatory agent (Li et al. 2013) for long, and commonly used as a substitute of Aconiti Radix and Aconiti Kusnezoffii Radix (Wan et al. 2007). Due to years of overharvesting and anthropogenic disturbances, the wild resources of A. vilmorinianum have reduced dramatically. Therefore, effective conser
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