Isolation and characterization of microsatellites for the neotropical dioecious palm Chamaedorea tepejilote (Arecaceae)
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Isolation and characterization of microsatellites for the neotropical dioecious palm Chamaedorea tepejilote (Arecaceae) and cross‑amplification in other Chamaedorea species Eric J. Fuchs1,3 · Alfredo Cascante‑Marin1 · Ruth Madrigal‑Brenes1 · Nick Harvey2 · Mauricio Quesada3,4 Received: 5 March 2020 / Accepted: 10 June 2020 © Springer Nature B.V. 2020
Abstract Palms are important components of tropical and subtropical ecosystems and have even been considered keystone plant resources that can support a large array of pollinators and frugivores. Palms are also economically important. Chamaedorea tepejilote Liebm. is a widely distributed palm with important bioeconomic potential for food, traditional medicine and ornamental purposes. Eighteen microsatellite primers were developed for C. tepejilote. Polymorphism and genetic diversity were evaluated in 71 individuals from four populations in Costa Rica. Thirteen loci were polymorphic and the number of alleles in the pooled sample ranged between 5 and 20, the average number of alleles was 10.61. Average observed heterozygosity was Ho = 0.607 ± 0.04 (SD) and the average expected heterozygosity was He = 0.600 ± 0.03. The exclusion probability of the combined 13 loci, was PE = 0.998. We tested transferability of the markers in the congeneric C. costaricana, C. pinnantifrons and C. macrospadix. Dioecious species are common in tropical forests; however, few studies have analyzed gene flow patterns in these species. The markers developed for C. tepejilote are an important tool to quantify gene flow patterns and the distribution of genetic diversity within populations. This information will be useful for the development of conservation and management practices of this dioecious tropical palm species. Keywords Microsatellites · Dioecious species · Costa Rica · Heterozygosity · Genetic diversity
Introduction Palms are important components of tropical and subtropical ecosystems [1] and are among the oldest flowering monocotyledons [2]. They play an integral role in ecological communities because they provide resources for pollinators and frugivores. They have even been considered as keystone plant resources, as they support a large array of animals [3]. * Mauricio Quesada [email protected] 1
Escuela de Biología, Universidad de Costa Rica, San José 11501‑2060, Costa Rica
2
Genetic Marker Services, Brighton, UK
3
Laboratorio Nacional de Análisis y Síntesis Ecológica (LANASE), Escuela Nacional de Estudios Superiores Unidad Morelia, Universidad Nacional Autónoma de México, Morelia, Michoacán, Mexico
4
Instituto de Investigaciones en Ecosistemas y Sustentabilidad, Universidad Nacional Autónoma de México, Morelia, Michoacán, Mexico
Many palm species are also economically important and represent major sources of food, oils, fibers, cattle forage, thatching and are valuable for ornamental purposes [4]. Palms in the genus Chamaedorea are dioecious and shrubby understory plants restricted to moist and wet tropical forests of the Americas [
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