Characterization, the Antioxidant and Antimicrobial Activity of Exopolysaccharide Isolated from Poultry Origin Lactobaci
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Characterization, the Antioxidant and Antimicrobial Activity of Exopolysaccharide Isolated from Poultry Origin Lactobacilli Muhammad Shahid Riaz Rajoka 1,2 & Hafiza Mahreen Mehwish 3 & Hafiz Fakhar Hayat 4 & Nazim Hussain 5 & Sobia Sarwar 6 & Humaira Aslam 7 & Ahmad Nadeem 8 & Junling Shi 2 Published online: 3 December 2018 # Springer Science+Business Media, LLC, part of Springer Nature 2018
Abstract The natural antioxidant agent is urgently needed to prevent the negative effects of newly generated free radicals and chronic disorders. Recently, the microbial exopolysaccharide (EPS) is currently used as a potential biopolymer due to its unique biological characteristics. In this study, the biological potential was carried out on the EPSs produced by Lactobacillus reuteri SHA101 (EPS-lr) and Lactobacillus vaginalis SHA110 (EPS-lvg) isolated from gut cecum samples of healthy poultry birds (hen). As results, the EPS-lr and EPS-lvg showed the emulsifying activity of 37.8 ± 1.6% and 27.8 ± 0.5% after the 360 h, respectively. The scanning electron microscopy analysis of EPS-lr and EPS-lvg demonstrated a smooth surface with a compact structure. The both EPSs exhibited strong antibacterial activity against E. coli and Salmonella typhimurium in vitro. In additions, at 4 mg/mL concentration, the EPS-lr and EPS-lvg samples showed potent antioxidant activity regarding hydroxyl radical DPPH (2,2-diphenyl-1-picrylhydrazyl) radical, superoxide anion radical and reducing power at OD700 nm. Furthermore, the EPS-lr and EPS-lvg (600 μg/mL) possessed antitumor activity against colon cancer (Caco-2) cell after 72 h. The results suggested that these EPSs would have great potential in the application of antitumor and antioxidant foods, biomedicine, and pharmaceutics. Keywords Lactobacillus reuteri . Lactobacillus vaginalis . Poultry birds . Exopolysaccharide . Antioxidant . Anticancer
Introduction * Muhammad Shahid Riaz Rajoka [email protected]; [email protected] 1
Department of Food Science and Engineering, College of Chemistry and Chemical Engineering, Shenzhen University, Shenzhen, Guangdong, People’s Republic of China
2
Key Laboratory for Space Bioscience and Space Biotechnology, School of Life Sciences, Northwestern Polytechnical University, Xi’an 710072, Shaanxi, People’s Republic of China
3
Department of Pharmacy, School of Medicine, Key Laboratory of Novel Health Care Product, Engineering Laboratory of Shenzhen Natural Small Molecules Innovative Drugs, Shenzhen University, Shenzhen, Guangdong, People’s Republic of China
4
Institute of Microbiology, University of Agriculture, Faisalabad, Pakistan
5
Center for Applied Molecular Biology, University of Punjab, Lahore, Pakistan
6
Department of Pharmacy, University of Agriculture, Faisalabad, Pakistan
7
Muhammad Medical College, Mirpur Khas, Pakistan
8
Nuclear Institute for Agriculture and Biology, Faisalabad, Pakistan
Among the exopolysaccharide (EPS)-producing microorganisms, lactic acid bacteria (LAB) have attained special interest due to the extraordinary
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