Isolation and Characterization of Pseudomonas sp. Cr13 and Its Application in Removal of Heavy Metal Chromium
- PDF / 918,211 Bytes
- 10 Pages / 595.276 x 790.866 pts Page_size
- 14 Downloads / 210 Views
Isolation and Characterization of Pseudomonas sp. Cr13 and Its Application in Removal of Heavy Metal Chromium Dafei Gong1 · Fengcai Ye1 · Caoping Pang1 · Zujun Lu1 · Changhua Shang1 Received: 8 May 2020 / Accepted: 7 August 2020 © Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract The purpose of this study was to elaborate the characteristics of Pseudomonas sp. Cr13, including physiological and biochemical characteristics, optimization of growth conditions, minimum inhibitory concentration of Cr6+ and resistance to other heavy metals, removal efficiency of Cr6+, and antibiotics sensitivity. A strain Pseudomonas sp. Cr13 was screened from mine d2+ (50 mg l−1). The optimum contaminated soils, which could tolerate high concentration of C r6+ (up to 250 mg l−1) and C pH, NaCl concentration, and temperature for growth were 6, 10% NaCl, and 30 °C, respectively. The removal efficiency of Cr6+ by strain Pseudomonas sp. Cr13 was studied. The removal efficiency of C r6+ decreased with the increased concentra6+ tion of Cr . Under the optimal conditions, the maximum of the removal rate can reach up to 94.26% in contaminated soils. In addition, antibiotics sensitivity of this strain was investigated. It was found that this strain was sensitive to nine types of antibiotics, which would lay a good foundation for the choice of selective marker in genetic engineering modification of this strain. The results in this article would lay a good foundation for the bioremediation of heavy metals pollution in the future. Pseudomonas sp. Cr13 can tolerate high concentration of C r6+ and partially remove C r6+, which make Cr13 an attractive option for the bioremediation of heavy metal chromium (Cr). Our findings suggest that Pseudomonas sp. Cr13 is a potential bacterium with the ability of bioremediation of heavy metal Cr.
Introduction Human activities and high costs of remediation of contaminated environment resulted in many polluted sites [1]. Harmful effects of heavy metals are enriched throughout the food chains [2]. The previous studies suggested that there was a severe effect of heavy metals on human health through food * Changhua Shang [email protected] Dafei Gong [email protected] Fengcai Ye [email protected] Caoping Pang [email protected] Zujun Lu [email protected] 1
College of Life Science, Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection, Ministry of Education, Guangxi Normal University, No. 1, Yanzhong Road, Yanshan District, 541006 Guilin, Guangxi, China
chain [3–5]. The mining and smelting plants were one of the main sources of heavy metals contamination with the most severe risk to human health. Heavy metals pollution primarily occurred in the site of southern China such as Dachang town, Nandan county, Hechi city of Guangxi autonomous region, which is known as “China’s tin capital.” The heavy metals such as Cd, Cr, and Hg were the predominant and serious pollutants. Heavy metals could significantly affect the microbial communities
Data Loading...
