Isolation and Identification of Two Brucella Species from a Volcanic Lake in Mexico
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Isolation and Identification of Two Brucella Species from a Volcanic Lake in Mexico Lesset Del Consuelo Ramos‑Ramírez1 · Zeus Saldaña‑Ahuactzi2 · Laura Morales‑Lara3 · Ygnacio Martínez‑Laguna2 · Elsa Iracena Castañeda‑Roldán1,2 Received: 30 January 2020 / Accepted: 25 August 2020 © Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract Brucellosis is a zoonosis caused by bacteria of the Brucella genus. Any source of contamination that could be infectious must be monitored to reduce the risk of exposure to brucellosis, so the purpose of this work was to determine the presence of Brucella spp. on surface water and tilapia (Oreochromis niloticus) skin from a volcanic lake in Mexico. A seasonal sampling during 2016–2017 was carried out at fifteen specific sites for water sampling and five sites for the collection of tilapia fish. From all water and fish samples tested, we found only three isolates of Brucella species. We isolated and identified B. abortus from surface water through bacteriological and molecular techniques, and B. abortus and B. suis from the same tilapia skin sample. The isolated strains likely came from breeding animals that are common to the region, such as infected pigs or cattle with Brucella abortus or B. suis, respectively. A similar finding has not been reported in a water from volcanic lake or tilapia fish in Mexico. We concluded that B. abortus and B. suis are present on the surface water of the volcanic lake and tilapia skin as possible contaminants derived from biological material from cows and pigs carrying this bacterium.
Introduction
Electronic supplementary material The online version of this article (doi:https://doi.org/10.1007/s00284-020-02184-z) contains supplementary material, which is available to authorized users. * Elsa Iracena Castañeda‑Roldán [email protected] Lesset Del Consuelo Ramos‑Ramírez [email protected] Zeus Saldaña‑Ahuactzi [email protected] Laura Morales‑Lara [email protected] Ygnacio Martínez‑Laguna [email protected] 1
Posgrado en Ciencias Ambientales, Instituto de Ciencias, Benemérita Universidad Autónoma de Puebla, 72570 Puebla, Mexico
2
Centro de Investigaciones en Ciencias Microbiológicas, Instituto de Ciencias Y Benemérita Universidad Autónoma de Puebla, 72570 Puebla, Puebla, Mexico
3
Facultad de Ciencias Químicas, Departamento Bioquímica – Alimentos, Benemérita Universidad, 72570 Puebla, Puebla, Mexico
Brucellosis is an infectious disease caused by bacteria of the Brucella genus transmitted from animals to humans [1]. The disease is transmitted from animals to humans via ingestion (unpasteurized milk or dairy products), inhalation, or exposure through the conjunctiva or skin abrasions [1, 2]. Brucellosis predominates in Mediterranean countries, the Middle East, and Latin America [3]. Based on the host preference and pathogenicity, twelve species are recognized within the genus Brucella: B. melitensis, B. abortus, B. suis, B. canis, B. ovis, B. neotomae. B. ceti, B. pinnipediae, B. micro
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