Isolation and molecular identification of industrially important enzyme producer yeasts from tree barks and fruits
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ORIGINAL PAPER
Isolation and molecular identification of industrially important enzyme producer yeasts from tree barks and fruits H. Tansel Yalçın1 · Begüm Fındık1 · Yiğit Terzi1 · Ebru Uyar2 · Fatima Shatila1 Received: 21 July 2020 / Revised: 27 September 2020 / Accepted: 19 October 2020 © Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract This study aimed to identify the yeast strains associated with the tree bark samples collected from the Aegean and Marmara regions and from rotten fruit samples. Fifty-one yeast strains were successfully isolated and screened for their abilities to produce industrially important extracellular enzymes. Thirty isolates demonstrated ability to produce at least two different enzymes and were selected for subsequent molecular identification using sequence analysis of ITS region and D1/D2 domain of the 26S rDNA. The most prevalent strains belonged to Papiliotrema laurentii (%23), Papiliotrema terrestris (%13) and Candida membranifaciens (%10). Papiliotrema laurentii and Papiliotrema terrestris recorded the highest enzymatic activities for all the screened enzymes. To the best of our knowledge, this is the first report that identifies the yeast strains associated with the tree barks of Turkey and among the limited comprehensive studies that screened considerable number of isolates for their ability to produce several industrially important enzymes. Keywords Tree bark · Yeast isolation · Extracellular enzyme screening · Molecular identification · ITS-PCR · D1/D2 domain
Introductıon The majority of industrial enzymes are of microbial origin. This can be partly attributed to their ability to produce high concentrations of extracellular enzymes (Brahmachari et al. 2017). Moreover, they confer advantage over their animal or plant counterparts as their isolation process is easier, fermentation cycles are shorter and can be grown on cheap culture media. Recombinant DNA technology facilitated enhanced microbial enzyme production which consequently gained wider interest and range of applications in pharmaceuticals, food, feed, agriculture, chemicals, medicine and energy. Other industries that made use of enzymes include Communicated by Erko Stackebrandt. * H. Tansel Yalçın [email protected] 1
Faculty of Science, Biology Department, Basic and Industrial Microbiology Section, Ege University, Izmir, Turkey
Faculty of Arts and Sciences, Biology Department, Molecular Biology Section, Harran University, Sanliurfa, Turkey
2
cosmetics, textile, detergents, leather as well as pulp and paper industries (Brahmachari et al. 2017; Arora et al. 2020). Today, more than 500 commercial products are produced by enzyme-catalyzed reactions. Additionally, they have been also employed in environmenally friendly processes, such as biodegradation and waste treatment (Singh et al. 2016; Arora et al. 2020). Based on their wide range of applications, the market share of these biocatalysts is expected to grow from $ 5.5 billion in 2018 to $ 7.0 billion in 2023 with compound ann
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