Phaeotremella camelliae sp. nov. (Phaeotremellaceae, Tremellales), A Novel Yeasts Isolated from Tea-Oil Fruits in Jiangx
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Phaeotremella camelliae sp. nov. (Phaeotremellaceae, Tremellales), A Novel Yeasts Isolated from Tea‑Oil Fruits in Jiangxi Province, China Yong Sun1 · Gui‑Shuang Wang2,3 · Ai‑Hua Li4 · Wangmu2 · Xian‑Qiu Chui1 · Ji‑Hong Jiang1 · Qi‑Ming Wang3,5 Received: 29 September 2019 / Accepted: 26 May 2020 © Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract Sixty-two isolates among the 65 yeast strains isolated from Jiangxi province, China, were identified into 15 known species based on the sequence analysis of the D1/D2 domains of the LSU rRNA and ITS region. The other three strains, GaoanZ14, GaoanC57, and GaoanC191, isolated from tea-oil fruits, were identified as two undescribed species of Phaeotremella based on the multiple gene sequence analysis, physiological, and biochemical comparisons. Strains GaoanC57 and GaoanC191 had one substitution difference both in the D1/D2 domains of the LSU rRNA and ITS region. They formed a separate branch from the other Phaeotremella species in the D1/D2 and multiple genes trees, and differed from the known species by at least 10 nucleotide substitutions in the D1/D2 domains and more than 6% mismatches in the ITS region. The phylogenetic analysis indicated that those two strains represent a novel species of Phaeotremella, for which the names Phaeotremella camelliae sp. nov. (Holotype CGMCC 2.6141, Mycobank MB832699) is proposed. Only one strain, GaoanZ14, represents the other undescribed species of Phaeotremella, so it will be described in latter when more strains are found.
Introduction The order Tremellales is the largest known group in the class Tremellomycetes, which is a heterogeneous group showing a high diversity in both morphology and life cycles [1, 2]. It comprises teleomorphic species, for example Tremella spp., and anamorphic species, such as yeast species of Bullera, Cryptococcus and Fellomyces [1–5]. The family Phaeotremellaceae, with the type genus Phaeotremella, was proposed in the Tremellales by Liu et al. based on the * Ji‑Hong Jiang [email protected] 1
The Key Laboratory of Biotechnology for Medicinal Plant of Jiangsu Province, Jiangsu Normal University, Xuzhou 221116, Jiangsu, China
2
Tibet Agriculture & Animal, Husbandry University, Nyingchi 860000, Tibet, China
3
State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Science, Beijing 100101, China
4
China General Microbiological Culture Collection Center and State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China
5
College of Life Sciences, Hebei University, Baoding 071002, Hebei, China
multiple gene phylogenetic analyses [6]. According to the phylogenetic analyses, five Tremella species (T. foliacea, T. mycetophiloides, T. mycophaga, T. neofoliacea and T. simplex) and two Cryptococcus species (C. fagi and C. skinneri) were transferred to the genus Phaeotremella [6]. Spirin et al. revised the taxonomy of the Phaeotremella foliacea complex based on morphological, ecological, g
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