Isolation and validation of microsatellite markers from a depleted South African sciaenid species, the dusky kob ( Argyr
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TECHNICAL NOTE
Isolation and validation of microsatellite markers from a depleted South African sciaenid species, the dusky kob (Argyrosomus japonicus), by means of the FIASCO/454 approach L. Mirimin • J. C. Ruiz Guajardo • J. Vervalle Aletta Bester-Van der Merwe • S. Kerwath • B. Macey • P. Bloomer • R. Roodt-Wilding
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Received: 15 March 2013 / Accepted: 26 March 2013 / Published online: 26 April 2013 Ó Springer Science+Business Media Dordrecht 2013
Abstract The dusky kob (Argyrosomus japonicus) is a large, estuarine-dependent sciaenid fish that has been severely depleted in South African waters and that, in recent years, has received considerable attention from the local fish farming industry. Discovery and application of appropriate molecular markers is necessary to improve the understanding of wild population structure, assist the effectiveness of broodstock and breeding programmes, and ensure monitoring of potential interactions between wild and farmed fish. The present study uses a recently tested approach that combines the FIASCO enrichment protocol with 454 GS-FLX Next Generation Sequencing, to identify large numbers of microsatellite-containing sequences at a low cost and high discovery rate from the dusky kob genome. Following the FIASCO enrichment (targeting specifically tetranucleotide repeats), 2,355 potential tetranucleotide microsatellites (perfect repeat motifs including eight or more repeat units flanked by regions for primer design) were identified from 1/5th of a single 454 lane.
L. Mirimin (&) J. C. Ruiz Guajardo J. Vervalle A. Bester-Van der Merwe R. Roodt-Wilding Department of Genetics, Stellenbosch University, Private Bag X1, Matieland 7602, South Africa e-mail: [email protected] S. Kerwath B. Macey Department of Agriculture, Forestry and Fisheries, Private Bag X2, Rogge Bay 8012, South Africa S. Kerwath Department of Zoology, University of Cape Town, Private Bag X3, Rondebosch 7700, South Africa P. Bloomer Department of Genetics, University of Pretoria, Private Bag X2, Hatfield 0028, South Africa
From these sequences, a test panel of 60 potential markers was selected for validation. A total of eight (13 %) markers were successfully amplified from a test sample of wild dusky kob individuals and showed high levels of polymorphism (observed heterozygosity per locus ranging between 0.375 and 0.905). Cross-species amplification of seven of these markers was also successfully carried out in another closely related and commercially important South African sciaenid species, the silver kob (A. inodorus). The microsatellite markers developed in the present study are readily available tools suitable to address genetic variability of Argyrosomus species of southern Africa. Keywords FIASCO 454 GS-FLX Microsatellites Sciaenid Argyrosomus spp
The dusky kob (Argyrosomus japonicus Temminck and Schlegel 1843) is a marine fish species that occurs in estuarine and coastal waters of southern Africa, between the Cape of Good Hope and Mozambique (Griffiths and Heemstra 1995). Poor managemen
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