Isolation and characterisation of sixteen microsatellite markers cross-amplifying in a complex of three African agricult
- PDF / 156,894 Bytes
- 4 Pages / 595.276 x 790.866 pts Page_size
- 63 Downloads / 203 Views
TECHNICAL NOTE
Isolation and characterisation of sixteen microsatellite markers cross-amplifying in a complex of three African agricultural pests (Ceratitis rosa, C. anonae and C. fasciventris, Diptera: Tephritidae) H. Delatte • M. Virgilio • C. Simiand S. Quilici • M. De Meyer
•
Received: 6 July 2012 / Accepted: 7 July 2012 / Published online: 27 July 2012 Ó Springer Science+Business Media B.V. 2012
Abstract Sixteen new polymorphic microsatellite loci were developed for the three species of the Ceratitis FAR complex (C. rosa, C. fasciventris and C. anonae) and their optimal amplification conditions characterised. These markers were tested on six field populations of the three fruit flies sampled in different African countries. After Bonferroni corrections, allele frequencies supported Hardy–Weinberg equilibrium for most of the loci, with only two loci not being at the equilibrium in all populations. No linkage disequilibrium was detected among all pairs of loci tested. These new markers will be a useful tool to study the intra- and interspecific genetic structure of these African agricultural pests and will help disentangling their well-known classification issues. Keywords Tephritidae FAR complex Agricultural pests Microsatellites
Fruit flies are Diptera of the Tephritidae family and include some of the world’s most serious agricultural pests. They cause billions of dollars of losses to a wide variety of fruit, vegetable and flower crops including citrus, tomato, olives and mango (White and Elson-Harris 1992). The Natal fruit fly, Ceratitis rosa, is a major African agricultural pest attacking a wide variety of host-fruits distributed across more than 25 plant families (Copeland et al. 2006;
H. Delatte (&) C. Simiand S. Quilici CIRAD, UMR PVBMT, 97410 Saint-Pierre, La Re´union, France e-mail: [email protected] M. Virgilio M. De Meyer Royal Museum for Central Africa, Leuvensesteenweg 13, 3080 Tervuren, Belgium
De Meyer et al. 2002). C. rosa is part of the so called ‘‘Ceratitis FAR complex’’ (Barr et al. 2006; De Meyer 2005) which also includes the two morphologically similar but less aggressive pests C. fasciventris and C. anonae (De Meyer 2005). Recent molecular analyses based on mitochondrial and nuclear markers failed to resolve the three morphospecies of the complex (Virgilio et al. 2008) so that their taxonomic status and genetic structure is still not clearly characterised. By using a 454 pyrosequencing technology developed by Genoscreen (www.genoscreen.fr) we developed and tested a set of 16 polymorphic microsatellite markers cross-amplifying in the three species. These markers represent a useful tool that will be used in the molecular characterisation of the intra- and interspecific structuring of the complex. Fly samples of each of the three species targeted were collected from 11 localities in Africa. Samples of C. rosa were from: Mozambique, Kenya and South Africa, samples of C. fasciventris from: Kenya, Rwanda, Zambia and Benin, and samples of C. anonae from: Kenya, Democratic Rep
Data Loading...
