Isolation, Identification, Antibacterial Effects of Antibiotic Drugs, and Chinese Herbal Extracts to the Pathogenic Bact
The pathogenic bacteria was isolated from Turbot (Scophthalmus maximus) with swollen abdomen, and proved to be the pathogenic bacteria by the infection experiment. In this research, VITEK-2 compact system was used to identify the pathogenic bacteria, and
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Isolation, Identification, Antibacterial Effects of Antibiotic Drugs, and Chinese Herbal Extracts to the Pathogenic Bacteria of Swollen Abdomen from Scophthalmus maximus in Vitro Xuan Wu, Dongqing Bai, Guoxia Zhu, Yanbin Ji, Zhichao Jia and Peng Zhou Abstract The pathogenic bacteria was isolated from Turbot (Scophthalmus maximus) with swollen abdomen, and proved to be the pathogenic bacteria by the infection experiment. In this research, VITEK-2 compact system was used to identify the pathogenic bacteria, and test the antibacterial effects of antibiotic drugs, paper diffusion method was used to evaluate the antibacterial effects of three kinds of Chinese herbal extracts on the pathogenic bacteria of swollen abdomen from Turbot in Vitro.The results showed that the pathogenic bacteria of swollen abdomen from Turbot was stenotrophomonas maltophilia, and the separation purity was 99 %. Without Trimethoprim, S. maltophilia had no CLSI salient point to the antibiotics of Ampicillin, Amikacin, Aztreonam, Cefazolin, Cefepime, Cefuroxime, Cefuroxime Axetil, Cefotetan, Ceftazidime, Ceftriaxone, Ciprofloxacin, Piperacillin, Imipenem, Gentamicin, Levofloxacin, Nitrofurantoin, Sulbactam, Tazobactam. Extract of the scutellaria and honeysuckle had different antibacterial effects to the S. maltophilia, and then the best bacteriostatic (MIC) and bactericidal (MBC) effects were 3.125 mg/mL and 6.25 mg/mL, respectively. However, the best MIC and MBC effects to extract from polygonum cuspidate were 100 mg/mL and 200 mg/mL, MIC and MBC effects lower than that extract from the scutellaria and honeysuckle. The results can provide reference materials for the clinical use of aquaculture and aquatic animal health cultivation.
Keywords Turbot (Scophthalmus maximus) Stenotrophomonas maltophilia Isolation and identification Antimicrobial susceptibility Chinese herbal medicine
X. Wu D. Bai (&) G. Zhu Y. Ji Z. Jia P. Zhou Tianjin Key Lab of AQUA-Ecology and Aquaculture, Department of Fishery Science, Tianjin Agricultural University, Tianjin 300384, People’s Republic of China e-mail: [email protected]
T.-C. Zhang et al. (eds.), Proceedings of the 2012 International Conference on Applied Biotechnology (ICAB 2012), Lecture Notes in Electrical Engineering 250, DOI: 10.1007/978-3-642-37922-2_79, Ó Springer-Verlag Berlin Heidelberg 2014
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79.1 Introduction Turbot (S. maximus) is one of the most economically important marine cultured fish in the world. The fish mainly distributes in the west of Pacific Ocean. At present, Turbot has become an important commercial species to the industrialized culture in the coastal areas of north of Chinese. But with high density of culture, disease outbreaks have occurred and caused heavy economic losses. Swollen abdomen is the main disease in the Turbot. It was a comprehensive disease that can cause infections to most of the aquatic organisms. The diseased fishes appeared slightly with enlargement of liver and spleen and obviously bloated abdomen. The anatomy of t
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