Study on Antibacterial Activity of Radix isatidis Extracts and Preliminary Investigation of Their Antibacterial Mechanis
The antibacterial mechanism of Radix isatidis extracts against Escherichia coli and Bacillus subtilis was investigated by determining the changes in electrical conductivity of bacterial suspension treated by Radix isatidis extracts, as well as the changes
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Study on Antibacterial Activity of Radix isatidis Extracts and Preliminary Investigation of Their Antibacterial Mechanism Lin Tian and Zhan Wang Abstract The antibacterial mechanism of Radix isatidis extracts against Escherichia coli and Bacillus subtilis was investigated by determining the changes in electrical conductivity of bacterial suspension treated by Radix isatidis extracts, as well as the changes of phosphorus metabolism and protein expression. The results showed that the electrical conductivity of treated bacterial suspension was significantly increased, indicating that Radix isatidis extracts could damage the integrity of cell membrane. In addition, the consumption of phosphorus was decreased in the Radix isatidis-treated bacteria, which influenced the synthesis of important cellular components, such as phospholipid, energy metabolism. Coomassie brilliant blue G-250 colorimetric assay demonstrated that Radix isatidis extracts could block the expression of some proteins in bacterial cells.
Keywords Antibacterial mechanism Bacillus subtilis Escherichia coli Radix isatidis
180.1 Introduction Chemical preservatives are widely used in the food industry in recent years. Although it can effectively inhibit bacterial growth, chemical preservatives have some side effects, teratogenic, carcinogenic, or mutagenic effect to harm human health, as well as a threat to the natural environment. Therefore, people pay more attention to the research and development of easy to use, effective and nontoxic natural food preservatives [1, 2].
L. Tian (&) Z. Wang Department of Basic Science, Tianjin Agricultural University, Tianjin 300384, People’s Republic of China e-mail: [email protected]
T.-C. Zhang et al. (eds.), Proceedings of the 2012 International Conference on Applied Biotechnology (ICAB 2012), Lecture Notes in Electrical Engineering 251, DOI: 10.1007/978-3-642-37925-3_180, Ó Springer-Verlag Berlin Heidelberg 2014
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China is the birthplace of Chinese herbal medicine and the largest production country. Chinese herbal medicine attracted much of the world’s attention because of its unique concept and effects on disease. Its effect and safety has been confirmed in the long history. Herbal medicine used as a food preservative, not only to avoid the toxic hazards of chemical preservatives, but also can give the food a unique flavor. Chinese herbal medicine as a welcomed and promising preservative has the following advantages: First, because it is a natural substance, it has no toxic side effects within the normal range of use; Second, it has multiple effects, such as health care, antibacterial, and disease prevention [3]. Radix Isatidis has been widely applied in the research and development of Chinese medicines. However, the research on Radix Isatidis antibacterial mechanism has not been found. In this study, we investigated the antibacterial mechanism of Radix Isatidis extracts liquid against Escherichia coli and Bacillus subtilis in order to provide a theoretical su
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