Isotope Internal Standard Method for Determination of Four Acrylamide Compounds in Food Contact Paper Products and Food
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Isotope Internal Standard Method for Determination of Four Acrylamide Compounds in Food Contact Paper Products and Food Simulants by Ultra-High Performance Liquid Chromatography Tandem Mass Spectrometry Cong Zhang 1,2,3 & Yanping Xian 1 & Xindong Guo 1 & Huidi Liu 1 & Hao Dong 1 & Zhiqing Xun 1 & Jinfeng Huang 1 & Xiang Feng 3
Received: 23 August 2015 / Accepted: 19 November 2015 / Published online: 26 November 2015 # Springer Science+Business Media New York 2015
Abstract A new method was developed for the determination of four acrylamide compounds (acrylamide, methacrylamide, N-methylol acrylamide, N-(Methoxymethyl)methacrylamide) in food contact paper products, three kinds of water-based food simulants, and dry food simulant (modified polyphenylene oxide, MPPO) by using ultra-high performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS). Acetonitrile was used as the extraction solvent for different kinds of samples. The extraction solution of paper products was purified with QuEChERS technology. Four analytes were separated by gradient elution in a UPLC HSS T3 column (100 mm×2.1 mm, 1.8 μm) with methanol and 0.1 % formic acid water as mobile phases, and then detected in electrospray ionization mode of MS/MS with multiple reaction monitoring (MRM). Under the optimal conditions, the calibration curves for four analytes were linear within the range of 1.0–200 μg/L and the correlation coefficients were higher than 0.998. The quantitation limits of the method (S/N=10) of four analytes were in the range of 0.3–20 μg/kg. The mean recoveries for five sample matrixes at three spiked concentration levels of 0.3–200 μg/kg were in the range of 81–108 % with the relative
* Xindong Guo [email protected] * Hao Dong [email protected] 1
Guangzhou Quality Supervision and Testing Institute, Guangzhou510000, China
2
School of Public Health, Sun Yat-sen University, Guangzhou 510080, China
3
Manufacture License Review Service Center of Guangdong Province, Guangzhou 510620, China
standard deviations (RSDs, n=6) values ranging from 2.5 to 7.1 %. The developed method is accurate, simple and rapid, which can be applied to the determination of acrylamide compounds in food contact paper products and food simulants. Keywords Acrylamide compounds . Food contact paper products . Food simulants . Ultra-high performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) . Isotope internal standard method
Introduction N-nitro acrylamide (AM), methacrylamide (AAM), N-methylol acrylamide (HAM), and N-(Methoxymethyl)methacrylamide, as shown in Fig. 1, are important chemical raw materials. AM is commonly used in the synthesis of polyacrylamide (PAM) and HAM is a widely-used cross-linking monomer. In paper industry, AM, PAM, AAM, HAM, and N-(Methoxymethyl) methacrylamide can polymerized with some other monomers (e.g., acrylonitrile, acrylic acid and acrylate) to form copolymers, which can be used as paper-reinforcing agents, dispersing agents, surface treatment agents, and paper pipe adhesives (Kim et al. 2015; Omar et al.
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