Jabuticaba peel extract modulates adipocyte and osteoblast differentiation of MSCs from healthy and osteoporotic rats
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ORIGINAL ARTICLE
Jabuticaba peel extract modulates adipocyte and osteoblast differentiation of MSCs from healthy and osteoporotic rats Alann Thaffarell Portilho Souza1 · Gileade Pereira Freitas1 · Helena Bacha Lopes1 · Gabriela Guaraldo Campos Totoli1 · Adriana Gadioli Tarone2 · Mario Roberto Marostica‑Junior2 · Adalberto Luiz Rosa1 · Marcio Mateus Beloti1 Received: 10 July 2020 / Accepted: 20 August 2020 © The Japanese Society Bone and Mineral Research and Springer Japan KK, part of Springer Nature 2020
Abstract Introduction The jabuticaba peel extract (JPE) contains bioactive compounds that regulate fat metabolism. Because the negative correlation between fat accumulation and bone formation in bone marrow, we hypothesized that JPE inhibits adipocyte as well as favors osteoblast differentiation of mesenchymal stromal cells (MSCs) under healthy and osteoporotic conditions, a disease that display an imbalance between adipocyte and osteoblast differentiation resulting in reduced bone mass. Material and methods To test these hypotheses, bone marrow MSCs were harvested from healthy and osteoporotic rats and cultured in adipogenic and osteogenic media with three concentrations of JPE, 0.25, 5 and 10 µg/ml, and vehicle (control). After selecting the most efficient concentrations of JPE, we used them to evaluate adipocyte and osteoblast differentiation of MSCs from both sources. Results We observed that, in general, JPE inhibited adipocyte differentiation of MSCs with more pronounced effects in cells from healthy than osteoporotic rats. In addition, JPE increased osteoblast differentiation, exhibiting a slightly higher osteogenic potential on MSCs from osteoporotic compared to healthy condition. Conclusion Our results demonstrated that JPE drives MSCs to inhibit adipocyte differentiation and toward osteoblast differentiation under healthy and osteoporotic conditions. These findings pave the way for further translational studies to investigate the therapeutic possibilities of JPE in both prevention and treatment of osteoporosis. Keywords Adipocyte · Jabuticaba · Mesenchymal stromal cell · Osteoblast · Osteoporosis
Introduction Products of natural origin have been increasingly used as a viable alternative to treat several diseases, including those that cause damage to bone tissue [1–3]. The raw materials of these products are easily found in nature, such as fruits, vegetables, herbs and nuts, the peels, roots, leaves and stems Alann Thafarell Portilho Souza and Gileade Pereira Freitas contributed equally to this work. * Marcio Mateus Beloti [email protected] 1
Bone Research Lab, School of Dentistry of Ribeirão Preto, University of São Paulo, Av Do Café S/N, Ribeirão Preto, SP 14040‑904, Brazil
School of Food Engineering, University of Campinas, Rua Monteiro Lobato 80, Campinas 13083‑862, SP, Brazil
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of which are used due to their beneficial properties [4, 5]. The main advantages that make natural products a safe and affordable alternative compared to synthetic drugs currently available are na
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