Kinesin-7 CENP-E regulates the formation and structural maintenance of the acrosome
- PDF / 36,908,140 Bytes
- 16 Pages / 595.276 x 790.866 pts Page_size
- 90 Downloads / 148 Views
REGULAR ARTICLE
Kinesin-7 CENP-E regulates the formation and structural maintenance of the acrosome Zhen-Yu She 1,2
&
Kai-Wei Yu 1,2 & Ya-Lan Wei 3,4 & Ning Zhong 1,2 & Yang Lin 1,2
Received: 13 June 2020 / Accepted: 5 November 2020 # Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract The acrosome is a special organelle that develops from the Golgi apparatus and the endolysosomal compartment in the spermatids. Centromere protein E (CENP-E) is an essential kinesin motor in chromosome congression and alignment. This study is aimed at investigating the roles and mechanisms of kinesin-7 CENP-E in the formation of the acrosome during spermatogenesis. Male ICR mice are injected with GSK923295 for long-term inhibition of CENP-E. Chemical inhibition and siRNA-mediated knockdown of CENP-E are carried out in the GC-2 spd cells. The morphology of the acrosomes is determined by the HE staining, immunofluorescence, and transmission electron microscopy. We have identified CENP-E is a key factor in the formation and structural maintenance of the acrosome during acrosome biogenesis. Long-term inhibition of CENP-E by GSK923295 results in the asymmetric acrosome and the dispersed acrosome. CENP-E depletion leads to the malformation of the Golgi complex and abnormal targeting of the PICK1- and PIST-positive Golgi-associated vesicles. Our findings uncover an essential role of CENP-E in membrane trafficking and structural organization of the acrosome in the spermatids during spermatogenesis. Our results shed light on the molecular mechanisms involved in vesicle trafficking and architecture maintenance of the acrosome. Keywords Kinesin-7 . CENP-E . Acrosome . The Golgi complex . Spermatogenesis
Introduction Acrosome biogenesis is a crucial step in the differentiation of a spermatid into a spermatozoon. The defects in acrosome development result in severe abnormalities in spermatozoa, which are related with globozoospermia and male infertility Zhen-Yu She, Kai-Wei Yu and Ya-Lan Wei contributed equally to this work. * Zhen-Yu She [email protected] 1
Department of Cell Biology and Genetics, The School of Basic Medical Sciences, Fujian Medical University, Fuzhou 350122, Fujian, China
2
Key Laboratory of Stem Cell Engineering and Regenerative Medicine, Fujian Province University, Fuzhou 350122, Fujian, China
3
Fujian Obstetrics and Gynecology Hospital, Fuzhou 350011, Fujian, China
4
Medical Research Center, Fujian Maternity and Child Health Hospital, Affiliated Hospital of Fujian Medical University, Fuzhou 350001, Fujian, China
(Dam et al. 2007; Guidi et al. 2018; Khawar et al. 2019). In the spermatids, acrosome biogenesis consists of four phases, including the Golgi phase, the cap phase, the acrosome phase, and the maturation phase (Clermont and Leblond 1955; Da Costa et al. 2019). The maturation of the acrosome is mainly fulfilled by the anterograde trafficking and fusion of Golgiderived vesicles from the trans-Golgi network to the anterior section of the nucleus (Da Costa et al. 2019). However, molecular
