Laccase isoform diversity on basal medium in Cyathus bulleri and role in decolorization/detoxification of textile dyes a
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(2020) 36:164
ORIGINAL PAPER
Laccase isoform diversity on basal medium in Cyathus bulleri and role in decolorization/detoxification of textile dyes and effluent A. Vats1 · S. Mishra2 Received: 3 June 2020 / Accepted: 20 September 2020 © Springer Nature B.V. 2020
Abstract Laccases (EC 1.10.3.2) are multi-copper oxidases that can degrade several xenobiotics, including textile dyes. Present study investigated the nature of laccase isoforms induced by 2,6-dimethylaniline in Cyathus bulleri cultivated on basal salt medium. Two isoforms, LacI and LacII were identified and purified by a combination of ultrafiltration and ion-exchange chromatography. The MS spectrum of the two proteins displayed a number of non-identical and identical molecular peaks (m/z), and, the latter were mapped to protein originating from the previously reported Laccase (Lcc) 1 gene. The LacI isoform exhibited higher catalytic efficiency (Kcat/Km) towards 2,2’-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid), 2,6-dimethoxyphenol, guaiacol and pyrogallol and was tolerant to high levels of chloride ions and resistant to EDTA. Higher decolorization of several dyes such as Direct Scarlet B (67%), Reactive Brilliant blue-R (96%), Direct Orange 34 (50%) and Reactive Red198 (95%) by the LacI isoform makes it a good candidate for degradation of synthetic dyes. The decolorization of Direct Orange 34 by laccases is being reported for the first time. Many of the properties exhibited by this isoform make it a good candidate for large scale production and applications for use in the dyeing industry. Graphic Abstract
Keywords Cyathus bulleri · Dye detoxification · Effluent treatment · Laccase isoforms
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s11274-020-02939-7) contains supplementary material, which is available to authorized users. * S. Mishra [email protected] A. Vats [email protected] 1
Department of Chemical and Environmental Engineering, University of Nottingham, Nottingham NG7 2RD, UK
Department of Biochemical Engineering and Biotechnology, Indian Institute of Technology Delhi, Hauz‑Khas, New Delhi 110016, India
2
Introduction Laccases (EC 1.10.3.2) are copper containing oxidoreductases that catalyse the oxidation of a wide range of substrates, mainly phenolics, methoxy-substituted monophenols and aromatic amines (Bourbonnais et al. 1995). Thus, these find useful applications in delignification of lignocellulosic biomass, bleaching in pulp and paper industries, clarification of juice and wine as well as in organic synthesis of valueadded products, biofuels and biosensors (Mate and Alcalde 2016). In recent years, laccases have been widely applied for decolorizing complex textile dyes and effluents (Zucca
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et al. 2015). Secreted as a part of ‘ligno-cellulose’ degrading enzyme cluster by fungal organisms, their application in dye removal processes is favoured as these are green catalysts and depend only on molecular oxygen unli
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