Hyperstabilization of a thermophile bacterial laccase and its application for industrial dyes degradation
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Hyperstabilization of a thermophile bacterial laccase and its application for industrial dyes degradation Julián E. Gianolini1,2 · Claudia N. Britos1 · Carlos B. Mulreedy1 · Jorge A. Trelles1,2 Received: 6 March 2020 / Accepted: 25 May 2020 © King Abdulaziz City for Science and Technology 2020
Abstract In the present study, a novel extracellular laccase isolated from Geobacillus stearothermophilus ATCC 10149 was entrapped in a bionanocomposite matrix consisting of copper alginate (Cu-alginate) supplemented with the nanoclay bentonite. After optimization, this nanobiocatalyst was able to degrade up to 90% of Remazol Brilliant Blue R (RBBR) without the addition of redox mediators and retained 70% of its initial activity for at least 1440 h, equivalent to more than 288 uses. The incorporation of nanoclay allowed alginate beads to be used in alkaline pH and strengthened its mechanical properties. Besides, this thermophilic laccase was able to decolorize other structurally different synthetic dyes such as Methyl Orange, Malachite Green and Indigo Carmine. These preliminary results suggested that the nanobiocatalyst could be a suitable option for dye decolorization and be further developed for large scale bioremediation of toxic dyes. Keywords Biodegradation · Geobacillus · Nanoclay · Bentonite · Cu-alginate · Immobilization
Introduction It is estimated that about 10–15% of dye used in textile industry is discharged into the aqueous ecosystem due to inadequacies during the dyeing process and inappropriate liberation of the effluents (Singh et al. 2017; Maniyam et al. 2018). Since synthetic dyes have complex aromatic structures, they are stable against light, oxidizing agents, pH and thermal fluctuations, ionic strength changes and metabolism of ubiquitous microbes, so they persist in the environment and become recalcitrant molecules (Vatandoostarani et al. 2018). Furthermore, dyes have proven to be toxic to the aquatic biota and some of them have been reported to be carcinogenic and mutagenic agents (Akpor 2018). Owing to the toxicological risk and environmental damage that dyes can present, their removal from industrial wastewater has been studied through the application of * Jorge A. Trelles [email protected] 1
Laboratory of Sustainable Biotechnology (LIBioS), National University of Quilmes, Roque Sáenz Peña 352, B1876BXD Bernal, Argentina
National Scientific and Technical Research Council (CONICET), Godoy Cruz 2290, C1425FQB CABA, Argentina
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several physico-chemical methods like adsorption, coagulation, sedimentation and chemical oxidation. However, the use of physical methods in wastewater treatment implies high costs and the implementation of these methods, especially adsorbents, generates large amounts of sludge. This requires adequate disposal, which is defiant since the adsorbents are still ingrained with dyes and stay toxic, leaving the problem without a solution (Akpor 2018; Maniyam et al. 2018). Biological treatment methods are more effective due to their high efficiency, cost ef
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