Leaf chlorophyll estimates of temperate deciduous shrubs during autumn senescence using a SPAD-502 meter and calibration
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RESEARCH PAPER
Leaf chlorophyll estimates of temperate deciduous shrubs during autumn senescence using a SPAD-502 meter and calibration with extracted chlorophyll Alison Donnelly 1 & Rong Yu 1,2 & Chloe Rehberg 1 & Gretchen Meyer 3 & Erica B. Young 4 Received: 17 February 2020 / Accepted: 12 March 2020 # INRAE and Springer-Verlag France SAS, part of Springer Nature 2020
Abstract & Key message The soil plant analysis development (SPAD) meter proved effective at estimating leaf Chl content of native and non-native temperate deciduous shrubs. However, due to the change in the relationship between SPAD values and Chl content during the course of autumn senescence, it may be necessary to establish separate calibration equations to cover a range of leaf Chl concentrations. & Context Non-destructive estimates of leaf chlorophyll (Chl) content by hand-held chlorophyll meters such as the Minolta SPAD-502 provide an effective time-efficient method of collecting field data on senescence.
This paper is dedicated to the memory of John Finnan, an outstanding agronomist. Handling Editor: Erwin Dreyer Contribution of the co-authors AD conceived of idea, designed experiment, collected data, performed chlorophyll extractions, carried out data analysis, and wrote manuscript. EBY collected data, defined chlorophyll extraction method, and commented on data analysis and manuscript in general. GM confirmed shrub identification and commented on data analysis and manuscript in general. CR helped with data collection and chlorophyll extractions and commented on manuscript. RY carried out statistical tests, advised on data analysis, and commented on manuscript in general. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s13595-020-00940-6) contains supplementary material, which is available to authorized users. * Alison Donnelly [email protected] Rong Yu [email protected] Chloe Rehberg [email protected]
Gretchen Meyer [email protected] Erica B. Young [email protected] Extended author information available on the last page of the article
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Annals of Forest Science
(2020) 77:30
& Aims To establish useable species-specific calibration equations of relationships between chlorophyll meter readings and leaf Chl content. & Methods During autumn, we collected SPAD values and quantum yield of photosystem-II (ΦPSII) over a 10-week period from 1044 leaf samples representing native and non-native temperate deciduous shrub species growing in the wild. Subsequently, Chl was extracted from leaf discs (1 cm diameter), incubated at 65 °C for 2 h in dimethyl sulfoxide, and quantified by spectroscopy. Relationships between extracted Chl and SPAD values were established using linear, quadratic, and exponential equations. & Results Quadratic functions proved the most reliable fit for the data. Interestingly, non-native species tended to have higher leaf Chl content and SPAD values but also exhibited higher variability than native species. The strength (r2) of the SPAD-Chl relationships was wea
