Light Emission Properties and Biological Applications of Lanthanide Doped Oxide Nanoparticles
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1064-PP02-07
Light Emission Properties and Biological Applications of Lanthanide Doped Oxide Nanoparticles Genevieve Mialon1, Domitille Giaume1, Melanie Moreau1, Didier Casanova2, Thanh-Liem Nguyen2, Michel-Robert Popoff3, Antigoni Alexandrou2, Thierry Gacoin1, and Jean-Pierre Boilot1 1 Laboratory of Condensed Matter Physics, Ecole Polytechnique, Palaiseau, 91128, France 2 Laboratory of Optics and Biosciences, Ecole Polytechnique, Palaiseau, 91128, France 3 Unité Bactéries Anaérobies et Toxines, Institut Pasteur, paris, 75724, France Light Emission Properties and Biological Applications of Lanthanide Doped Oxide Nanoparticles Genevieve Mialon1, Domitille Giaume1, Melanie Moreau1, Didier Casanova2, Thanh-Liem Nguyen2, Michel-Robert Popoff3, Antigoni Alexandrou2, Thierry Gacoin1, and Jean-Pierre Boilot1 1 Laboratory of Condensed Matter Physics, Ecole Polytechnique, Palaiseau, 91128, France 2 Laboratory of Optics and Biosciences, Ecole Polytechnique, Palaiseau, 91128, France 3 Unité Bactéries Anaérobies et Toxines, Institut Pasteur, Paris, 75724, France ABSTRACT Yttrium vanadate particles doped with europium are studied for their applications as biomolecule labels. Two parts of our recent work will be presented. The first concerns the thermal treatment of particles incorporated in a silica matrix. After annealing at 1000°C and redispersion in water by dissolution of the silica matrix, the structural and optical properties are greatly improved : without any modification of size, the obtained nanoparticles appear as perfect single crystals of 33 nm and have the same emission properties as the bulk material. Their quantum yield is 37% for 10% europium doping and the emission lifetime is 0.8 ms. The second aspect concerns the functionalization of the nanoparticles by the sol-gel condensation of aminopropyltriethoxysilane at the surface of particles. The chemical accessibility of amino groups grafted on the particles is measured by reaction with an organic fluorescent tag FITC (fluorescein isothiocyanate). These amine-coated nanoparticles were coupled to a peptidic toxin via a cross-linker and allowed observation of the toxin motion bound to its membrane receptor. INTRODUCTION Fluorescent organic compounds have been extensively used for the visualization of the different components of biological systems [1]. Now, an interesting issue is to track labeled individual species during in vitro or in vivo experiments. The use of organic dyes is limited for that purpose because of their rapid photobleaching. About ten years ago, the use of inorganic nanocrystals such as CdSe was suggested since they exhibit a bright and stable luminescence [2]. Many studies done in this field in the last years have indeed shown that these particles provide a new tool for biologists to study the action of biologically active species [3]. In this context, it appears also interesting to evaluate such applications in the case of rare earth doped oxides, which represent another class of inorganic luminescent nanoparticles. We have recently reviewed our
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