LINC00324 affects non-small cell lung cancer cell proliferation and invasion through regulation of the miR-139-5p/IGF1R
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LINC00324 affects non‑small cell lung cancer cell proliferation and invasion through regulation of the miR‑139‑5p/IGF1R axis Meiqing Zhang1 · Baoquan Lin1 · Yaming Liu1 · Tengfei Huang1 · Mengmeng Chen1 · Duohuang Lian1 · Shilong Deng1 · Congwen Zhuang1 Received: 26 April 2020 / Accepted: 27 June 2020 © Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract Long non-coding RNAs (lncRNAs) are proved to perform critical function in regulating cancer cell behavior. It is reported that LINC00324 promotes lung adenocarcinoma development by regulating miR-615-5p/AKT1 axis. This study aimed to demonstrate whether LINC00324 participates in non-small cell lung cancer (NSCLC) pathogenesis through other molecular mechanism. Relative mRNA, lncRNA, and microRNA levels were analyzed using quantitative real-time–polymerase chain reaction (qRT-PCR). Western blot was used to detect protein level. MTT assay shown proliferation ability and transwell assay shown invasive ability. Luciferase reporter assay illustrated the interaction between RNA molecules. In NSCLC, the high expression of LINC00324 had correlation with the poor prognosis. LINC00324 promoted the proliferation and invasion of NSCLC cells while miR-139-5p inhibited these behaviors. LINC00324 overexpression promoted insulin-like growth factor 1 receptor (IGF1R) expression via absorbing miR-139-5p. The tumor-promoting effects of LINC00324 were attenuated through miR-139-5p overexpression. Highly expressed LINC00324 in NSCLC through sponged miR-139-5p to elevate IGF1R expression and promoted cell proliferation and invasion. This research demonstrated that LINC00324 is a potential NSCLC diagnosis and therapy target. Keywords LINC00324 · miR-139-5p · IGF1R · NSCLC
Introduction Statistical data showed that lung cancer is prevalent and leads to enormous mortalities [1]. As a severe risk in human health, lung cancer belongs to malignant tumor and the abnormal cancer cell proliferation and metastasis cause the invasion and migration into adjacent tissue or the other organs [2]. Lung cancer is separated based on their unique pathological features, one called small cell lung cancer (SCLC) and the other called non-small cell lung cancer (NSCLC) [3]. NSCLC accounts for 85% of lung cancer [4]. As a consequence of the difficulties in lung cancer early
Meiqing Zhang and Baoquan Lin have contributed equally to this work. * Congwen Zhuang [email protected] 1
Cardiothoracic Surgery, 900 Hospital of the Joint Logistics Team, No.156 West Second‑beltway Road, Fuzhou 350025, Fujian, China
diagnosis, most patients has already had advanced lung cancer when diagnosed and the overall survival rate keeps at a low level [5]. Although the understanding of tumorigenesis principle has been progressed a lot, the discovery of novel target molecules in lung cancer makes a great contribution to the development of effective diagnostic and therapeutic strategies. RNA molecules larger than 200 nucleotides and without protein encoding function are called long non-coding RNA (lncRNA
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