LINC00472 regulates vascular smooth muscle cell migration and proliferation via regulating miR-149-3p
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RESEARCH ARTICLE
LINC00472 regulates vascular smooth muscle cell migration and proliferation via regulating miR-149-3p Ran Jing 1 & Wei Pan 1 & Tianyi Long 1 & Zhenyu Li 2 & Chuanchang Li 2 Received: 11 February 2020 / Accepted: 7 September 2020 # Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract LncRNAs are one group of gene modulators functioning via several mechanisms in pathological and physiological conditions. We noted that LINC00472 expression level is elevated in atherosclerotic coronary tissues compared with normal coronary artery samples. LINC00472 is also upregulated in vascular smooth muscle cells (VSMCs) induced by TNF-α and PDGF-BB. Ectopic expression of LINC00472 induced VSMC migration and proliferation. The predicted binding sequence between miR-149-3p and LINC00472 was analyzed by LncBase Predicted. Overexpression of miR-149-3p decreases the luciferase activity of wild-type reporter plasmid, but not the mutant one. Ectopic expression of LINC00472 suppresses the expression of miR-149-3p in VSMCs. Furthermore, we demonstrated that miR-149-3p expression is decreased in atherosclerotic coronary tissues. MiR-149-3p was downregulated in VSMCs induced by TNF-α and PDGF-BB. Overexpression of LINC00472 induces VSMC migration and proliferation via regulating miR-149-3p. These data suggested that LINC00472 acts a critical role in the migration and proliferation of VSMCs partly via modulating miR-149-3p. Keywords Air pollution . LINC00472 . lncRNA . miR-149-3p . VSMCs
Introduction Cardiovascular disease, including myocardial infarction, stroke, ischemic heart disease, and hypertension, remains the 1st cause of deaths worldwide (Lieberman et al. 2017; Pratte et al. 2017; Zhou et al. 2017b). Air pollution such as PM2.5 is the most frequent risk for cardiovascular disease including myocardial infarction, stroke, ischemic heart disease, and hypertension (Miller and Newby 2020; Zhang and Routledge 2020). Jerrett et al. (2009) indicated that higher concentrations of ozone or PM2.5 were significantly correlated with a
Responsible Editor: Mohamed M. Abdel-Daim * Chuanchang Li [email protected] 1
Cardiovascular Department, Xiangya Hospital, Central South University, Changsha City 410000, Hunan Province, China
2
Geriatric Department of Xiangya Hospital, Central South University, Changsha City 410000, Hunan Province, China
high risk of the death of cardiopulmonary causes. Tonne and Wilkinson (2013) found that mortality was higher among patients with exposure to the PM 2.5 in Wales and England. The development of cardiovascular disease is one result of atherosclerosis (Zhang 2009; Zhang et al. 2015; Zuo et al. 2015). The underlying mechanism of atherosclerosis is complex and remains not well understood (Wei et al. 2013; Xue et al. 2015). Abnormal proliferation and migration of vascular smooth muscle cells (VSMCs) following the injury of vessels is important to the development of atherosclerosis and intimal hyperplasia (Shin et al. 2005; Sun et al. 2013; Wang et al. 2012). Since VSMCs are the
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