LINC01006 facilitates cell proliferation, migration and invasion in prostate cancer through targeting miR-34a-5p to up-r
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Cancer Cell International Open Access
PRIMARY RESEARCH
LINC01006 facilitates cell proliferation, migration and invasion in prostate cancer through targeting miR‑34a‑5p to up‑regulate DAAM1 Enhui Ma1, Qianqian Wang2, Jinhua Li3, Xinqi Zhang4, Zhenjia Guo1 and Xiaofeng Yang5*
Abstract Background: Prostate cancer (PCa) is a kind of malignancy occurring in the prostate gland. Substantial researches have proved the major role of long noncoding RNAs (lncRNAs) in PCa. However, the role of long intergenic non-protein coding RNA 1006 (LINC01006) in PCa has not been investigated yet. Methods: RT-qPCR was used to examine the expression levels of LINC01006 and its downstream targets. The function of LINC01006 in PCa was tested by in vitro and in vivo assays. With application of RNA pull down, RNA immunoprecipitation (RIP) and luciferase reporter assays, the interaction among LINC01006, miR-34a-5p and disheveled associated activator of morphogenesis 1 (DAAM1) were verified. Results: LINC01006 expression presented high in PCa cell lines. LINC01006 silencing suppressed cell proliferative, migratory, invasive capacities while accelerated apoptotic rate. Besides, LINC01006 knockdown also suppressed tumor growth and metastasis in vivo. Furthermore, miR-34a-5p, a tumor suppressor in PCa, was sponged by LINC01006. Moreover, DAAM1 was targeted by miR-34a-5p and promoted PCa progression. More intriguingly, rescue assays suggested that the inhibitory effect of LINC01006 knockdown on PCa development was offset by DAAM1 overexpression. Conclusions: LINC01006 promoted PCa progression by sponging miR-34a-5p to up-regulate DAAM1, providing a novel target for PCa therapy. Keywords: LINC01006, miR-34a-5p, DAAM1, Prostate cancer Background Prostate cancer (PCa) is recognized as a type of malignancy for elderly males all over the world [1]. PCa ranks as the third most common deadly cancer on account of its high morbidity and mortality [2, 3]. At the same time, lack of biomarkers in early stage results in poor diagnosis of PCa. Thus, delving into the potential molecular
*Correspondence: [email protected] 5 Department of Urology, Zaozhuang Municipal Hospital, NO.41 Longtou Road, Shizhong District, Zaozhuang 277100, Shandong, China Full list of author information is available at the end of the article
mechanism in PCa for a deep understanding is of vital importance. Long noncoding RNAs (lncRNAs) are a group of transcripts greater than 200 nts in length with no proteincoding capacity [4]. For the last few years, quantities of lncRNAs have been reported to be crucial regulators in cellular processes of various cancers [5–7]. For instance, a novel lncRNA LSAMP-AS1 is involved in PCa process via targeting miR-183-5p/DCN axis [8]. LncRNA RHPN1AS1 has been claimed to accelerate the breast cancer development [9]. UCA1/miR-495/PRL-3 axis results in cell proliferation in gastric cancer [10]. LINC01006 was
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