LINC01094/miR-577 axis regulates the progression of ovarian cancer
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RESEARCH
Open Access
LINC01094/miR-577 axis regulates the progression of ovarian cancer Jing Xu1, Ping Zhang2* , Huajun Sun3 and Yang Liu4
Abstract Background: Long intergenic non-coding RNA 01094 (LINC01094) is probably a novel regulator in cancer biology. This study aimed to probe into the function and mechanism of LINC01094 in ovarian cancer (OC). Methods: Quantitative real-time polymerase chain reaction (qRT-PCR) assay was utilized to measure LINC01094 and miR577 expressions in OC tissues and cell lines. Western blot was used to examine the expressions of epithelial-mesenchymal transition (EMT)-related proteins, β-catenin, c-Myc and cyclin D1. Cell counting kit-8 (CCK-8) and Transwell assays were used to detect the proliferation, migration and invasion of SKOV3 and 3AO cells, respectively. Eventually, dual-luciferase reporter gene assay was employed to detect the regulatory relationship between miR-577 and LINC01094. Results: LINC01094 expression was elevated in OC tissues and cell lines. High LINC01094 expression was associated with higher FIGO stage, lymph node metastasis and the shorter overall survival rate in patients with OC. Meanwhile, LINC01094 knockdown inhibited OC cell proliferation, migration, invasion and EMT. In addition, miR-577 was demonstrated to be a direct downstream target of LINC01094 in OC and inhibition of miR-577 reversed the biological effects of LINC01094 knockdown on OC cells. Additionally, LINC01094 / miR-577 axis regulated the expressions of β-catenin, c-Myc and cyclin D1 in OC cells. Conclusion: LINC01094 promotes the proliferation, migration, invasion and EMT of OC cells by adsorbing miR-577. Keywords: LINC01094, miR-577, Ovarian cancer, Cancer progression
Introduction Ovarian cancer (OC) is one of the deadliest cancers among women worldwide, triggering about 151,900 deaths per year [1, 2]. Because of the lack of obvious symptoms at the early stage of the disease, most patients with OC have distant metastasis when being diagnosed, and the five-year survival rate is extremely low [3]. Therefore, it is of great significance to delve into the mechanism of OC tumorigenesis and progression and to find effective markers for early detection and therapeutic targets. Long non-coding RNA (LncRNA) has been one of the research hotspots in recent years. Composed of more than 200 nucleotides in length, it is a kind of non-coding RNAs * Correspondence: [email protected] 2 Department of Reproductive Medicine, Linyi People’s Hospital, Fenghuang Street No. 233, Hedong District, Linyi, Shandong Province, China Full list of author information is available at the end of the article
(ncRNAs) that cannot encode proteins [4, 5]. Accumulating evidence shows that lncRNAs participate in a variety of biological activities [6]. Importantly, it is found that multiple lncRNAs are abnormally expressed in cancer cells, which modulate carcinogenesis and cancer progression [7]. Exploring the biological functions of lncRNA in the development of OC can provide insights for its diagnosis and treatment. A r
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