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ipid Bilayer Membrane Arrays: Fabrication and Applications Xiaojun Han, Guodong Qi, Xingtao Xu and Lei Wang Abstract The lipid bilayer is one of the most important self-assembled structures in nature. In addition to compartmentalizing the cell, the lipid bilayer maintains many physical and biological characteristics of cell membranes, including lateral fluidity. It provides a wealth of opportunities for the study of membrane properties. Recently there has been an increasing interest in lipid bilayer arrays fabrication and their applications. In this review, the leading methods for creating lipid bilayer arrays are categorized as mechanical methods, pre-patterning substrate, direct UV patterning, direct blotting or stamping, polymer liftoff technique, robotic micro spotting, and microfluidics. The applications of bilayer arrays for cell adhesion and activation, lipid bilayer based 2D electrophoresis, and high-throughput binding assays are also presented.







Keywords Cell adhesion High-throughput binding assay Lipid bilayer arrays Photolithography




Abbreviations TR-PE D-291 NBD-PE Egg PC POPC NBD-PG

TR-BSA

Texas Red 1, 2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine 4-(4-(didecylamino) styryl)-N-methylpyridinium iodide (4-di10-ASP) (N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)-1,2-dihexadecanoylsnglycero-3-phosphoethanolamine, triethylammonium salt) Egg phosphatidylcholine 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine 1-Acyl-2-{12-[(7-nitro-2-1,3-benzoxadiazol-4yl)amino]lauroyl}-sn-glycero-3-[phospho-rac-(1-glycerol)] (ammonium salt) Bovine serum albumin labeled with Texas Red X-SE

X. Han (&)
G. Qi
X. Xu
L. Wang School of Chemical Engineering and Technology, Harbin Institute of Technology, No. 92, West Da-Zhi Street, Harbin 150001, China e-mail: [email protected]

122

X. Han et al.

Rh-PE DOPC Oregon Green-DHPE Marina Blue-DHPE DNP-cap-DPPE GPI PG PC PS DPPC NBD PS FITC DOTAP Biotin-cap DOPE Tm SAM PDMS AFM CFM

1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine-N(lissamine-rhodamine B sulfonyl) 1,2-dioleoyl-sn-glycero-3-phosphocholine Oregon Green labeled 1,2-dihexadecanoyl-sn-glycero-3phosphoethanolamine Marina Blue labeled 1,2-dihexadecanoyl-sn-glycero-3phosphoethanolamine 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine-N-[6[(2,4-dinitrophenyl)amino]hexanoyl] (ammonium salt) Glycan-phosphatidyl inositol Phosphatidylglycerol Phosphatidylcholine Phosphatidylserine 1,2-dipalmitoyl-sn-glycero-3-phosphocholine 1,2-dioleoyl-sn-glycero-3-phospho-L-serine-N-(7-nitro-21,3-benzoxadiazol-4-yl) (diammonium salt) Fluorescein isothiocyanate 1,2-dioleoyl-3-trimethylammoniumpropane (chloride salt) 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(cap biotinyl) (sodium salt) Phase transition temperature Self assembled monolayer Poly(dimethyl siloxane) Atomic force microscopy Continuous flow microspotter

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Introduction........................................................................................................................ Fabrication Methods of Lipid Bilayer Membrane Arrays ...........