Lipid metabolism in adipose tissue and liver from diet-induced obese rats: a comparison between Wistar and Sprague-Dawle
- PDF / 1,239,276 Bytes
- 12 Pages / 595.276 x 790.866 pts Page_size
- 64 Downloads / 173 Views
ORIGINAL ARTICLE
Lipid metabolism in adipose tissue and liver from diet-induced obese rats: a comparison between Wistar and Sprague-Dawley strains Jonatan Miranda 1,2 & Itziar Eseberri 1,2 & Arrate Lasa 1,2 & María P. Portillo 1,2 Received: 25 January 2018 / Accepted: 18 October 2018 / Published online: 9 November 2018 # University of Navarra 2018
Abstract Some researchers have proposed important variations in adipose tissue among different strains of rats and mice in response to a high-caloric (hc) diet, but data concerning the mechanisms underlying these differences are scarce. The aim of the present research was to characterize different aspects of triacylglycerol (TG) metabolism and clock genes between Sprague-Dawley and Wistar rats. For this purpose, 16 male Sprague-Dawley and 16 male Wistar rats were divided into four experimental groups (n = 8) and fed either a normal-caloric (nc) diet or a hc diet for 6 weeks. After sacrifice, liver and epididymal, perirenal, mesenteric, and subcutaneous adipose tissue depots were dissected, weighed and immediately frozen. Liver TG content was quantified, RNA extracted for gene expression analysis and fatty acid synthase enzyme activity measured. Two-way ANOVA and Student’s t test were used to perform the statistical analyses. Under hc feeding conditions, Wistar rats were more prone to fat accumulation in adipose tissue, especially in the epididymal fat depot, due to their increased lipogenesis and fatty acid uptake. By contrast, both strains of rats showed similarly fatty livers after hc feeding. Peripheral clock machinery seems to be a potential explanatory mechanism for Wistar and Sprague-Dawley strain differences. In conclusion, Wistar strain seems to be the best choice as animal model in dietary-induced obesity studies. Keywords High-caloric feeding . Obesity . Strain . Wistar . Sprague-Dawley . Rats
Introduction Nowadays, obesity is considered the most prevalent nutritional disease around the world [18]. The widespread occurrence of this health problem in humans means that there is a need to study its causes and physiopathology. To carry out these studies, it is important to find useful animal models that sufficiently mimic all aspects of this human disease. There are different approaches to obesity induction in laboratory animals, such as genetic manipulation, dietary treatments, or neuroendocrine alteration induction [11]. In the case of dietary treatments, high-fat diets that entail an increase in * María P. Portillo [email protected] 1
Nutrition and Obesity Group, Department of Nutrition and Food Science, Faculty of Pharmacy and Lucio Lascaray Research Institute, University of País Vasco (UPV/EHU), Vitoria, Spain
2
CIBEROBN Physiopathology of Obesity and Nutrition, Institute of Health Carlos III (ISCIII), Madrid, Spain
caloric intake are widely used. In addition to their obesogenic effects, these diets induce metabolic alterations which are very similar to those found in obese humans [31]. Regarding the choice of animals, rodents are considered as ess
Data Loading...
